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  4. Colicin-E1 Protein, E.coli (305a.a, His, Myc)

Colicin-E1 Protein, E.coli (305a.a, His, Myc)

Cat. No.: HY-P705948
Handling Instructions Technical Support

Colicin-E1, a channel-forming colicin, serves as a transmembrane toxin inducing cytoplasmic membrane depolarization and cellular energy dissipation. It is part of a broader class of polypeptide toxins synthesized by and active against Escherichia coli and related bacterial strains. Colicin-E1 Protein, E.coli (305a.a, His, Myc) is the recombinant E. coli-derived Colicin-E1 protein, expressed by E. coli, with N-10*His, C-Myc labeled tag.

For research use only. We do not sell to patients.

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Description

Colicin-E1, a channel-forming colicin, serves as a transmembrane toxin inducing cytoplasmic membrane depolarization and cellular energy dissipation. It is part of a broader class of polypeptide toxins synthesized by and active against Escherichia coli and related bacterial strains. Colicin-E1 Protein, E.coli (305a.a, His, Myc) is the recombinant E. coli-derived Colicin-E1 protein, expressed by E. coli, with N-10*His, C-Myc labeled tag.
Background

Colicin-E1, a representative of the channel-forming colicins, operates as a transmembrane toxin with the capability to induce depolarization of the cytoplasmic membrane, resulting in the dissipation of cellular energy. Belonging to the broader class of colicins, these polypeptide toxins are synthesized by and exhibit activity against Escherichia coli and closely related bacterial strains.
Species

E.coli
Source

E. coli
Tag

N-10*His;C-Myc
Accession

P02978 (M1-D304)
Molecular Construction
N-term
10*His
Colicin-E1 (M1-D304)
Accession # P02978
Myc
C-term
Protein Length

Full length
Synonyms
Colicin-E1
AA Sequence

METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINAD

Predicted Molecular Mass
38.2 kDa
Purity

≥ 85%, as determined by reducing SDS-PAGE.
Documentation
Handling Instructions (2659 KB)

Colicin-E1 Protein, E.coli (305a.a, His, Myc) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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