Colicin-E1 Protein, E.coli (Cell-Free, His, Twin-Strep)
Colicin-E1, a channel-forming colicin, serves as a transmembrane toxin inducing cytoplasmic membrane depolarization and cellular energy dissipation. It is part of a broader class of polypeptide toxins synthesized by and active against Escherichia coli and related bacterial strains. Colicin-E1 Protein, E.coli (Cell-Free, His, Twin-Strep) is the recombinant E. coli-derived Colicin-E1 protein, expressed by E. coli Cell-free, with N-10*His, C-Twin-Strep labeled tag.
- Species: E.coli
- Source: E. coli Cell-free
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Colicin-E1, a channel-forming colicin, serves as a transmembrane toxin inducing cytoplasmic membrane depolarization and cellular energy dissipation. It is part of a broader class of polypeptide toxins synthesized by and active against Escherichia coli and related bacterial strains. Colicin-E1 Protein, E.coli (Cell-Free, His, Twin-Strep) is the recombinant E. coli-derived Colicin-E1 protein, expressed by E. coli Cell-free, with N-10*His, C-Twin-Strep labeled tag.
Background
Colicin-E1, a representative of the channel-forming colicins, operates as a transmembrane toxin with the capability to induce depolarization of the cytoplasmic membrane, resulting in the dissipation of cellular energy. Belonging to the broader class of colicins, these polypeptide toxins are synthesized by and exhibit activity against Escherichia coli and closely related bacterial strains.
Technical Parameters
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Species E.coli
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Source E. coli Cell-free
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Tag N-10*His;C-Twin-Strep
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Accession
P02978 (M1-I522)
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Gene ID/
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Molecular Construction
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N-term
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10*His
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Colicin-E1 (M1-I522)
Accession # P02978 -
Twin-Strep
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C-term
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Protein Length
Full length
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Synonyms
CEACAM5; Cell Adhesion Molecule CEACAM5; Prev. CEA; Meconium Antigen 100; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 Isoform 5D; CD66e; Carcinoembryonic Antigen-Related Cell Adhesion Molecule 5 Isoform 3D; Carcinoembryonic Antigen Related Cell Adhesion Molecule 5; CD66e Antigen; CEA Cell Adhesion Molecule 5; Carcinoembryonic Antigen
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AA Sequence
METAVAYYKDGVPYDDKGQVIITLLNGTPDGSGSGGGGGKGGSKSESSAAIHATAKWSTAQLKKTQAEQAARAKAAAEAQAKAKANRDALTQRLKDIVNEALRHNASRTPSATELAHANNAAMQAEDERLRLAKAEEKARKEAEAAEKAFQEAEQRRKEIEREKAETERQLKLAEAEEKRLAALSEEAKAVEIAQKKLSAAQSEVVKMDGEIKTLNSRLSSSIHARDAEMKTLAGKRNELAQASAKYKELDELVKKLSPRANDPLQNRPFFEATRRRVGAGKIREEKQKQVTASETRINRINADITQIQKAISQVSNNRNAGIARVHEAEENLKKAQNNLLNSQIKDAVDATVSFYQTLTEKYGEKYSKMAQELADKSKGKKIGNVNEALAAFEKYKDVLNKKFSKADRDAIFNALASVKYDDWAKHLDQFAKYLKITGHVSFGYDVVSDILKIKDTGDWKPLFLTLEKKAADAGVSYVVALLFSLLAGTTLGIWGIAIVTGILCSYIDKNKLNTINEVLGI
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Molecular Weight
Approximately 62 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add 5-50% of glycerol (final concentration). Our default final concentration of glycerol is 50%. Customers could use it as reference.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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