Copy Link Print Information

Complement C3a Protein, Bovine (His-SUMO)

Customer Review

Based on 1 Customer Validation

Complement C3/C3a proteins play a key role in initiating the complement system, serving as substrates for C3 convertase in both the classical and alternative pathways. Upon activation, C3 undergoes proteolytic cleavage, yielding C3b, which is covalently bound to surface or immune aggregates. Complement C3a Protein, Bovine (His-SUMO) is the recombinant bovine-derived Complement C3/C3a protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

For research use only. We do not sell to patients.
  • Species: Bovine
  • Source: E. coli
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Complement C3a Protein, Bovine (His-SUMO) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

Complement C3/C3a proteins play a key role in initiating the complement system, serving as substrates for C3 convertase in both the classical and alternative pathways. Upon activation, C3 undergoes proteolytic cleavage, yielding C3b, which is covalently bound to surface or immune aggregates. Complement C3a Protein, Bovine (His-SUMO) is the recombinant bovine-derived Complement C3/C3a protein, expressed by E. coli , with N-His, N-SUMO labeled tag.

Background

C3 assumes a pivotal role in the initiation of the complement system, serving as a substrate for C3 convertase in both the classical and alternative pathways. Upon activation, C3 undergoes proteolytic cleavage, generating C3b, which can covalently bind to cell surface carbohydrates or immune aggregates through its reactive thioester moiety. Concurrently, the anaphylatoxin C3a is produced as a result of C3 degradation, functioning as a mediator in local inflammatory processes. C3a induces smooth muscle contraction, enhances vascular permeability, and stimulates histamine release from mast cells and basophilic leukocytes. Additionally, in chronic inflammation, C3a acts as a chemoattractant for neutrophils.

Technical Parameters

  • Species Bovine
  • Source E. coli
  • Tag N-His;N-SUMO
  • Accession

    Q2UVX4 (S747-Q953)

  • Gene ID
    280677  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His-SUMO
    • C3 (S747-Q953)
      Accession # Q2UVX4
    • C-term
  • Protein Length

    Full Length of Complement C3c alpha' Chain fragment 1 Chain

  • Synonyms

    C3; Complement Component 3; Complement C3; C3a Anaphylatoxin; CPAMD1; Prepro-C3; ARMD9; Epididymis Secretory Sperm Binding Protein Li 62p; C3a; Acylation-Stimulating Protein Cleavage Product; C3b; HEL-S-62p; C3 And PZP-Like Alpha-2-Macroglobulin Domain-Co

  • AA Sequence

    SDLDDDIIPEEDIISRSQFPESWLWTVIEDLKQADKNGISTKLMNVFLKDSITTWEILAVSLSDKKGICVADPYEVTVMQDFFIDLRLPYSVVRNEQVEIRAILYNYREAENLKVRVELLYNPAFCSLATAKKRHQQTITIPARSSVAVPYVIVPLKIGLHEVEVKAAVYNHFISDGVKKTLKVVPEGVRVNKTVAVRTLNPEHLGQ

  • Predicted Molecular Mass

    39.5 kDa

  • Molecular Weight

    Approximately 44 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of 10 mM Tris-HCl, 1 mM EDTA, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • Complement C3a Protein, Bovine (His-SUMO) Product Image
    1/16
  • Complement C3a Protein, Bovine (His-SUMO) Product Image