Cope1 Protein, Mouse (sf9, His, Strep)
Cope1 Protein, Mouse (sf9, His, Strep) is the recombinant mouse-derived Cope1 protein, expressed by Sf9 insect cells, with Strep & His tag.
- Species: Mouse
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Cope1 Protein, Mouse (sf9, His, Strep) is the recombinant mouse-derived Cope1 protein, expressed by Sf9 insect cells, with Strep & His tag.
Background
Cope1 is a cytosolic protein complex that binds to dilysine motifs and reversibly associates with Golgi non-clathrin-coated vesicles. It mediates biosynthetic protein transport from the ER, via the Golgi apparatus, up to the trans Golgi network. The complex is required for budding from Golgi membranes and is essential for the retrograde Golgi-to-ER transport of dilysine-tagged proteins. In mammals, Cope1 can only be recruited by membranes associated with ADP-ribosylation factors (ARFs), which are small GTP-binding proteins. The complex also influences Golgi structural integrity, as well as the processing, activity, and endocytic recycling of LDL receptors (By similarity).
Technical Parameters
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Species Mouse
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Source Sf9 insect cells
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Tag Strep;His
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Accession
O89079 (M1-A308)
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Gene ID59042
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Protein Length
Full Length
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Synonyms
COPE; Epsilon-Coat Protein; Coat Protein Complex I Subunit Epsilon; Coatomer Protein Complex, Subunit Epsilon; Epsilon-COP; Coatomer Protein Complex Subunit Epsilon; COPI Coat Complex Subunit Epsilon; Coatomer Epsilon Subunit; Coatomer Subunit Epsilon; Ep
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AA Sequence
MAPPVPGAVSGGSGEVDELFDVKNAFYIGSYQQCINEAQRVKLSSPEREVERDVFLYRAYLAQRKYGVVLDEIKPSSAPELQAVRMFAEYLASENQRDSIVLELDREMSRSVDVTNTTFLLMAASIYFHDQNPDAALRTLHQGDGLECMAMTIQILLKLDRLDLARKELKKMQDQDEDATLTQLATAWVNLAVGGEKLQEAYYIFQELADKCSPTLLLLNGQAACHSAQGRWETAEGVLQEALDKDSGHPETLINLIVLSQHLGKPPEVTNRYLSQLKDAHRAHPFIKEYQAKENDFDRLAMQYAPSA
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Predicted Molecular Mass
37.8 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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