CRBN Protein, Human (His)
Based on 1 publication(s) in Google Scholar
CRBN Protein, Human (His) is the recombinant human-derived CRBN, expressed by E. coli , with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
CRBN Protein, Human (His) is the recombinant human-derived CRBN, expressed by E. coli , with C-6*His labeled tag.
Background
CRBN serves as the substrate recognition component of a DCX (DDB1-CUL4-X-box) E3 ubiquitin-protein ligase complex, mediating ubiquitination and subsequent proteasomal degradation of target proteins like MEIS2, ILF2, or GLUL. Its proper degradation of key regulatory proteins is essential for normal limb outgrowth and fibroblast growth factor FGF8 expression. CRBN maintains presynaptic glutamate release and cognitive functions (e.g., memory and learning) by negatively regulating large-conductance calcium-activated potassium (BK) channels in excitatory neurons. It likely functions by modulating BK channel assembly and neuronal surface expression via interaction with KCNT1. By similarity, CRBN may also regulate anxiety-like behaviors through a BK channel-independent mechanism. In TLR4 signaling, CRBN acts as a negative regulator by binding TRAF6 and ECSIT, thereby inhibiting ECSIT ubiquitination—a critical step in the pathway.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
Q96SW2-1 (M1-L442)
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Gene ID51185
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Protein Length
Full Length of Isoform-1
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Synonyms
CRBN; MRT2; Prev. MRT2A; Protein X 0001; Protein Cereblon; Cereblon; Mental Retardation, Non-Syndromic, Autosomal Recessive, 2A
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AA Sequence
MAGEGDQQDAAHNMGNHLPLLPAESEEEDEMEVEDQDSKEAKKPNIINFDTSLPTSHTYLGADMEEFHGRTLHDDDSCQVIPVLPQVMMILIPGQTLPLQLFHPQEVSMVRNLIQKDRTFAVLAYSNVQEREAQFGTTAEIYAYREEQDFGIEIVKVKAIGRQRFKVLELRTQSDGIQQAKVQILPECVLPSTMSAVQLESLNKCQIFPSKPVSREDQCSYKWWQKYQKRKFHCANLTSWPRWLYSLYDAETLMDRIKKQLREWDENLKDDSLPSNPIDFSYRVAACLPIDDVLRIQLLKIGSAIQRLRCELDIMNKCTSLCCKQCQETEITTKNEIFSLSLCGPMAAYVNPHGYVHETLTVYKACNLNLIGRPSTEHSWFPGYAWTVAQCKICASHIGWKFTATKKDMSPQKFWGLTRSALLPTIPDTEDEISPDKVILCL
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Molecular Weight
Approximately 56-65 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
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It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (237 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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