1. Recombinant Proteins
  2. Others
  3. CREG1/CREG Protein, Mouse (HEK293, His)

CREG1/CREG Protein, Mouse (HEK293, His)

Cat. No.: HY-P7852
COA Handling Instructions

CREG1/CREG is a potential regulator in transcriptional control that antagonizes adenovirus E1A-induced activation, emphasizing its role in regulating cell growth and differentiation. The glycosylation-dependent interaction with IGF2R highlights the multifaceted nature of CREG1/CREG in the molecular mechanisms governing transcriptional control. CREG1/CREG Protein, Mouse (HEK293, His) is the recombinant mouse-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CREG1/CREG Protein, Mouse (HEK293, His) is 189 a.a., with molecular weight of 26-34 kDa.

For research use only. We do not sell to patients.

Protein Expression Service

Size Price Stock Quantity
Free Sample   Apply now
2 μg $35 In-stock
10 μg $80 In-stock
50 μg $250 In-stock
100 μg $425 In-stock
500 μg $1200 In-stock
> 100 μg   Get quote  

* Please select Quantity before adding items.

Top Publications Citing Use of Products
  • Biological Activity

  • Technical Parameters

  • Properties

  • Documentation

Description

CREG1/CREG is a potential regulator in transcriptional control that antagonizes adenovirus E1A-induced activation, emphasizing its role in regulating cell growth and differentiation. The glycosylation-dependent interaction with IGF2R highlights the multifaceted nature of CREG1/CREG in the molecular mechanisms governing transcriptional control. CREG1/CREG Protein, Mouse (HEK293, His) is the recombinant mouse-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag. The total length of CREG1/CREG Protein, Mouse (HEK293, His) is 189 a.a., with molecular weight of 26-34 kDa.

Background

The CREG1/CREG protein emerges as a potential regulator in the transcriptional control of cell growth and differentiation. Notably, it acts as an antagonist to transcriptional activation and cellular transformation induced by the adenovirus E1A protein. The transcriptional control activity associated with cell growth necessitates the interaction with IGF2R. CREG1/CREG forms homodimers, and its interaction with IGF2R is dependent on glycosylation. These characteristics underline the multifaceted nature of CREG1/CREG in molecular mechanisms involved in transcriptional control, emphasizing its potential significance in modulating cellular growth, differentiation, and responses to external stimuli. Further investigation is crucial to unravel the specific pathways and molecular events orchestrated by CREG1/CREG in these regulatory processes.

Biological Activity

Measured by its binding ability in a functional ELISA. Immobilized IGF-II R at 2 μg/mL (100 μL/well) can bind Biotinylated CREG. The ED50 for this effect is 17.01 ng/mL.

  • Measured by its binding ability in a functional ELISA. Immobilized IGF-II R at 2 μg/mL (100 μL/well) can bind Biotinylated CREG. The ED50 for this effect is 17.01 ng/mL.
Species

Mouse

Source

HEK293

Tag

C-6*His

Accession

O88668 (R32-Q220)

Gene ID

433375  [NCBI]

Molecular Construction
N-term
CREG1 (R32-Q220)
Accession # O88668
6*His
C-term
Synonyms
rMuProtein CREG1/CREG, His; CREG1; cellular repressor of E1A-stimulated genes; cellular repressor of E1A-stimulated genes 1CREG; protein CREG1
AA Sequence

RGGRDHGDWDVDRRLPPLPPREDGPRVARFVTHVSDWGSLATISTIKEVRGWPFADIISISDGPPGEGTGEPYMYLSPLQQAVSDLQENPEATLTMSLAQTVYCRNHGFDPQSPLCVHIMMSGTVTKVNKTEEDYARDSLFVRHPEMKHWPSSHNWFFAKLKISRIWVLDYFGGPKVVTPEEYFNVTLQ

Molecular Weight

26-34 kDa

Purity
  • Greater than 95% as determined by reducing SDS-PAGE.
Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4 or 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

CREG1/CREG Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

Your Recently Viewed Products:

Inquiry Online

Your information is safe with us. * Required Fields.

Product Name

 

Salutation

Applicant Name *

 

Email Address *

Phone Number *

 

Organization Name *

Department *

 

Requested quantity *

Country or Region *

     

Remarks

Bulk Inquiry

Inquiry Information

Product Name:
CREG1/CREG Protein, Mouse (HEK293, His)
Cat. No.:
HY-P7852
Quantity:
MCE Japan Authorized Agent: