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CREG1/CREG Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

CREG1/CREG is a potential regulator in transcriptional control that antagonizes adenovirus E1A-induced activation, emphasizing its role in regulating cell growth and differentiation. The glycosylation-dependent interaction with IGF2R highlights the multifaceted nature of CREG1/CREG in the molecular mechanisms governing transcriptional control. CREG1/CREG Protein, Mouse (HEK293, His) is the recombinant mouse-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for CREG1/CREG Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CREG1/CREG Protein, Mouse (HEK293, His)
  • CREG1/CREG Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

CREG1/CREG is a potential regulator in transcriptional control that antagonizes adenovirus E1A-induced activation, emphasizing its role in regulating cell growth and differentiation. The glycosylation-dependent interaction with IGF2R highlights the multifaceted nature of CREG1/CREG in the molecular mechanisms governing transcriptional control. CREG1/CREG Protein, Mouse (HEK293, His) is the recombinant mouse-derived CREG1/CREG protein, expressed by HEK293 , with C-6*His labeled tag.

Background

The CREG1/CREG protein emerges as a potential regulator in the transcriptional control of cell growth and differentiation. Notably, it acts as an antagonist to transcriptional activation and cellular transformation induced by the adenovirus E1A protein. The transcriptional control activity associated with cell growth necessitates the interaction with IGF2R. CREG1/CREG forms homodimers, and its interaction with IGF2R is dependent on glycosylation. These characteristics underline the multifaceted nature of CREG1/CREG in molecular mechanisms involved in transcriptional control, emphasizing its potential significance in modulating cellular growth, differentiation, and responses to external stimuli. Further investigation is crucial to unravel the specific pathways and molecular events orchestrated by CREG1/CREG in these regulatory processes.

Verified Bioactivity

Measured by its binding ability in a functional ELISA. Immobilized IGF-II R at 2 μg/mL (100 μL/well) can bind Biotinylated CREG. The ED50 for this effect is 17.01 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized IGF-II R at 2 μg/mL (100 μL/well) can bind Biotinylated CREG. The ED50 for this effect is 17.01 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-6*His
  • Accession

    O88668 (R32-Q220)

  • Gene ID
    433375  [NCBI]
  • Molecular Construction
    • N-term
    • CREG1 (R32-Q220)
      Accession # O88668
    • 6*His
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    CREG1; Cellular Repressor Of E1A-Stimulated Genes 1, Isoform CRA_a; Prev. CREG; Cellular Repressor Of E1A-Stimulated Genes; Protein CREG1; Cellular Repressor Of E1A Stimulated Genes 1; Cellular Repressor Of E1A-Stimulated Genes 1

  • AA Sequence

    RGGRDHGDWDVDRRLPPLPPREDGPRVARFVTHVSDWGSLATISTIKEVRGWPFADIISISDGPPGEGTGEPYMYLSPLQQAVSDLQENPEATLTMSLAQTVYCRNHGFDPQSPLCVHIMMSGTVTKVNKTEEDYARDSLFVRHPEMKHWPSSHNWFFAKLKISRIWVLDYFGGPKVVTPEEYFNVTLQ

  • Molecular Weight

    Approximately 25-35 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

1.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
2.Lyophilized from a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized IGF-II R at 2 μg/mL (100 μL/well) can bind Biotinylated CREG. The ED50 for this effect is 17.01 ng/mL.

  • CREG1/CREG Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for CREG1/CREG Protein, Mouse (HEK293, His)
  • Experimental Validation Results for CREG1/CREG Protein, Mouse (HEK293, His)
  • CREG1/CREG Protein, Mouse (HEK293, His) Product Image