2. Recombinant Proteins
  3. Cytokines and Growth Factors CD Antigens
  4. CSF & Receptors Macrophage CD Proteins Platelet CD Proteins Endothelial cell CD Proteins
  5. G-CSF R G-CSF R/CD114
  6. G-CSFR/CD114 Protein, Human (HEK293, His-Flag)

G-CSFR/CD114 Protein, Human (HEK293, His-Flag)

Cat. No.: HY-P700528
Handling Instructions

The G-CSFR/CD114 Protein, acting as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocytic maturation, playing a pivotal role in the proliferation, differentiation, and survival of neutrophilic lineage cells. Additionally, it may participate in cell surface adhesion or recognition events. The homodimeric receptor binds two CSF3 molecules and interacts with CEACAM1, which down-regulates the CSF3R-STAT3 pathway through the recruitment of PTPN6, leading to the dephosphorylation of CSF3R. G-CSFR/CD114 Protein, Human (HEK293, His-Flag) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293, with C-6*His, C-Flag labeled tag. The total length of G-CSFR/CD114 Protein, Human (HEK293, His-Flag) is 597 a.a., with molecular weight of 69.8 kDa.

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Description

The G-CSFR/CD114 Protein, acting as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocytic maturation, playing a pivotal role in the proliferation, differentiation, and survival of neutrophilic lineage cells. Additionally, it may participate in cell surface adhesion or recognition events. The homodimeric receptor binds two CSF3 molecules and interacts with CEACAM1, which down-regulates the CSF3R-STAT3 pathway through the recruitment of PTPN6, leading to the dephosphorylation of CSF3R. G-CSFR/CD114 Protein, Human (HEK293, His-Flag) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293, with C-6*His, C-Flag labeled tag. The total length of G-CSFR/CD114 Protein, Human (HEK293, His-Flag) is 597 a.a., with molecular weight of 69.8 kDa.
Background

G-CSFR/CD114, the receptor for granulocyte colony-stimulating factor (CSF3), holds a pivotal role in orchestrating granulocytic maturation by governing the proliferation, differentiation, and survival of cells along the neutrophilic lineage. This homodimeric receptor engages with two CSF3 molecules, forming a crucial interaction that regulates essential cellular processes. Beyond its involvement in cellular maturation, G-CSFR/CD114 may contribute to adhesion or recognition events at the cell surface. Furthermore, its interaction with CEACAM1 serves as a regulatory axis, down-regulating the CSF3R-STAT3 pathway by recruiting PTPN6, which subsequently dephosphorylates CSF3R and modulates cellular signaling dynamics.
Species

Human
Source

HEK293
Tag

C-6*His;C-Flag
Accession

Q99062 (E25-P621)
Gene ID
Molecular Construction
N-term
CSF3R (E25-P621)
Accession # Q99062
6*His-Flag
C-term
Synonyms
colony stimulating factor 3 receptor (granulocyte); CD114; granulocyte colony-stimulating factor receptor; GCSFR; G-CSF-R; CD114 antigen; G-CSF receptor;
AA Sequence

ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTP

Molecular Weight

69.8 kDa
Purity

Greater than 90% as determined by reducing SDS-PAGE.
Endotoxin Level

<1 EU/μg, determined by LAL method.
Documentation
Handling Instructions (2659 KB)

G-CSFR/CD114 Protein, Human (HEK293, His-Flag) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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