CTRB1 Protein, Human (HEK293, His)
Chymotrypsinogen B (CTRB1) is the precursor of the pancreatic proteolytic enzymes. CTRB1 is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate the functional enzyme. CTRB1 enables serine-type endopeptidase activity, being involved in response to apoptotic signal, nutrient, cytokine and peptide hormone. CTRB1 is located in lysosome and is a biomarker of pancreatitis. CTRB1 Protein, Human (HEK293, His) is the recombinant human-derived CTRB1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Chymotrypsinogen B (CTRB1) is the precursor of the pancreatic proteolytic enzymes. CTRB1 is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate the functional enzyme. CTRB1 enables serine-type endopeptidase activity, being involved in response to apoptotic signal, nutrient, cytokine and peptide hormone. CTRB1 is located in lysosome and is a biomarker of pancreatitis. CTRB1 Protein, Human (HEK293, His) is the recombinant human-derived CTRB1 protein, expressed by HEK293 , with C-6*His labeled tag.
Chymotrypsinogen B (CTRB1) is a member of the serine protease family of enzymes and forms a principal precursor of the pancreatic proteolytic enzymes. CTRB1 is synthesized in the acinar cells of the pancreas and secreted into the small intestine where it undergoes proteolytic activation to generate the functional enzyme. CTRB1 enables serine-type endopeptidase activity, being involved in response to apoptotic signal, nutrient, cytokine and peptide hormone. CTRB1 induces apoptosis through Bid cleavage activity, as the truncated Bid can in turn cause rapid mitochondrial release of cytochrome c. CTRB1 is located in lysosome and is a biomarker of pancreatitis. CTRB1 gene encodes distinct isoforms, which may undergo similar processing to generate the mature protein[1][2][3][4][5].
Assay Procedure
Materials
Assay buffer: 0.1 M Tris-HCl, 1 mM CaCl2, 0.05% TWEEN 20, pH 8.0
Test protein: CTRB1 protein, human (HEK293, His) (HY-P70005)
Substrate: Suc-AAPF-pNA
Standard: p-Nitroaniline
Procedure
1. Dilute p-nitroaniline (standard) with assay buffer to concentrations of 0, 1.171875, 2.34375, 4.6875, 9.375, 18.75, 37.5, 75, 150, and 300 μM.
2. Add 100 µL of each concentration of the standard to the wells.
3. Measure the OD410 values using a microplate reader.
4. Plot a standard curve with the molarity of the standard on the y-axis and the measured OD on the x-axis to obtain the standard curve equation.
5. Dilute the substrate to 500 µM using the assay buffer.
6. Add 50 µL of 100 µg/mL human CTRB1 at different concentrations to the wells, then add 50 µL of 500 µM substrate to initiate the reaction. For the control group, add 50 µL of assay buffer and 50 µL of 500 µM substrate.
7. On a microplate reader, read for 5 minutes in kinetic mode at an OD410 wavelength.
8. Calculate the specific activity:
Specific Activity (pmol/min/μg) = | Adjusted Abs* (OD) x Conversion Factor ** (pmol/OD) |
| Incubation time (min) x amount of enzyme (μg) |
*Adjusted for Control
**Derived using calibration standard
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P17538 (C19-N263)
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Molecular Construction
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N-term
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CTRB1 (C19-N263)
Accession # P17538 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
CTRB1; Chymotrypsinogen B; Prev. CTRB; Chymotrypsinogen B1; Chymotrypsin
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AA Sequence
CGVPAIHPVLSGLSRIVNGEDAVPGSWPWQVSLQDKTGFHFCGGSLISEDWVVTAAHCGVRTSDVVVAGEFDQGSDEENIQVLKIAKVFKNPKFSILTVNNDITLLKLATPARFSQTVSAVCLPSADDDFPAGTLCATTGWGKTKYNANKTPDKLQQAALPLLSNAECKKSWGRRITDVMICAGASGVSSCMGDSGGPLVCQKDGAWTLVGIVSWGSDTCSTSSPGVYARVTKLIPWVQKILAAN
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Predicted Molecular Mass
26.9 kDa
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Molecular Weight
Approximately 28 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (236 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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