D15 Protein, Escherichia phage T5
D15 Protein, Escherichia phage T5 is the recombinant D15, expressed by E. coli , with tag Free labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
D15 Protein, Escherichia phage T5 is the recombinant D15, expressed by E. coli , with tag Free labeled tag.
Background
D15 is an enzyme with both 5'-exonucleolytic and structure-specific endonucleolytic activities, catalyzing the hydrolysis of DNA branched nucleic acid molecules and likely playing a role in viral genome replication. It is active on flap structures (branched duplex DNA containing a free single-stranded 5'-end), 5' overhangs, and pseudo-Y structures. The substrates require a free single-stranded 5' end, with endonucleolytic hydrolysis occurring at the junction of double- and single-stranded DNA. This function may be used, for example, to trim such branched molecules generated by Okazaki fragment synthesis during replication. by similar: The enzyme's function resembles that of other nucleases with similar activities.
Technical Parameters
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Species Others
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Source E. coli
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Tag Tag Free
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Accession
P06229 (M1-Q291)
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Gene ID2777611
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Protein Length
Full Length
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Synonyms
Flap endonuclease; EC:3.1.11.-; FEN; 5'-3' exonuclease; Exodeoxyribonuclease ; EC:3.1.11.3; D15; exo5; T5.130; T5p128
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AA Sequence
MSKSWGKFIEEEEAEMASRRNLMIVDGTNLGFRFKHNNSKKPFASSYVSTIQSLAKSYSARTTIVLGDKGKSVFRLEHLPEYKGNRDEKYAQRTEEEKALDEQFFEYLKDAFELCKTTFPTFTIRGVEADDMAAYIVKLIGHLYDHVWLISTDGDWDTLLTDKVSRFSFTTRREYHLRDMYEHHNVDDVEQFISLKAIMGDLGDNIRGVEGIGAKRGYNIIREFGNVLDIIDQLPLPGKQKYIQNLNASEELLFRNLILVDLPTYCVDAIAAVGQDVLDKFTKDILEIAEQ
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Predicted Molecular Mass
33.5 kDa
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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