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DAG1 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

DAG1 Proteinas, a pivotal extracellular glycoprotein, is crucial for laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Serving as a receptor for laminin-G domain-containing extracellular matrix proteins, DAG1 interacts with laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells and acts as a receptor for laminin LAMA5. DAG1 Protein, Human (HEK293, His) is the recombinant human-derived DAG1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DAG1 Protein, Human (HEK293, His)
  • DAG1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

DAG1 Proteinas, a pivotal extracellular glycoprotein, is crucial for laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. Serving as a receptor for laminin-G domain-containing extracellular matrix proteins, DAG1 interacts with laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells and acts as a receptor for laminin LAMA5. DAG1 Protein, Human (HEK293, His) is the recombinant human-derived DAG1 protein, expressed by HEK293 , with C-His labeled tag.

Background

Dystroglycan (DAG1) plays a crucial role in various biological processes, participating in laminin and basement membrane assembly, sarcolemmal stability, cell survival, peripheral nerve myelination, nodal structure, cell migration, and epithelial polarization. As an extracellular peripheral glycoprotein, DAG1 serves as a receptor for extracellular matrix proteins containing laminin-G domains. Specifically, it acts as a receptor for laminin-2 (LAMA2) and agrin in peripheral nerve Schwann cells, and also functions as a receptor for laminin LAMA5.

Verified Bioactivity

Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells. The ED50 for this effect is 1.377 μg/mL, corresponding to a specific activity is 726.216 units/mg.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells. The ED50 for this effect is 1.377 μg/mL, corresponding to a specific activity is 726.216 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    Q14118 (H30-V749)

  • Gene ID
    1605  [NCBI]
  • Molecular Construction
    • N-term
    • DAG1 (H30-V749)
      Accession # Q14118
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    DAG1; Alpha-Dystroglycan; Dystroglycan 1; Beta-Dystroglycan; 156DAG; Dystroglycan; AGRNR; Dystrophin; A3a; LGMDR16; DAG; MDDGA9; Dystroglycan 1 (Dystrophin-Associated Glycoprotein 1); MDDGC7; Dystrophin-Associated Glycoprotein-1; MDDGC9; Dystrophin-Associ

  • AA Sequence

    HWPSEPSEAVRDWENQLEASMHSVLSDLHEAVPTVVGIPDGTAVVGRSFRVTIPTDLIASSGDIIKVSAAGKEALPSWLHWDSQSHTLEGLPLDTDKGVHYISVSATRLGANGSHIPQTSSVFSIEVYPEDHSELQSVRTASPDPGEVVSSACAADEPVTVLTVILDADLTKMTPKQRIDLLHRMRSFSEVELHNMKLVPVVNNRLFDMSAFMAGPGNAKKVVENGALLSWKLGCSLNQNSVPDIHGVEAPAREGAMSAQLGYPVVGWHIANKKPPLPKRVRRQIHATPTPVTAIGPPTTAIQEPPSRIVPTPTSPAIAPPTETMAPPVRDPVPGKPTVTIRTRGAIIQTPTLGPIQPTRVSEAGTTVPGQIRPTMTIPGYVEPTAVATPPTTTTKKPRVSTPKPATPSTDSTTTTTRRPTKKPRTPRPVPRVTTKVSITRLETASPPTRIRTTTSGVPRGGEPNQRPELKNHIDRVDAWVGTYFEVKIPSDTFYDHEDTTTDKLKLTLKLREQQLVGEKSWVQFNSNSQLMYGLPDSSHVGKHEYFMHATDKGGLSAVDAFEIHVHRRPQGDRAPARFKAKFVGDPALVLNDIHKKIALVKKLAFAFGDRNCSTITLQNITRGSIVVEWTNNTLPLEPCPKEQIAGLSRRIAEDDGKPRPAFSNALEPDFKATSITVTGSGSCRHLQFIPVVPPRRVPSEAPPTEVPDRDPEKSSEDDV

  • Molecular Weight

    Approximately 95-110 kDa & 55-95 kDa & 17-20 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by the ability of the immobilized protein to enhance the adhesion of H4 human neuroglioma cells. The ED50 for this effect is 1.377 μg/mL, corresponding to a specific activity is 726.216 units/mg.

  • DAG1 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DAG1 Protein, Human (HEK293, His)
  • DAG1 Protein, Human (HEK293, His) Product Image