DDC Protein, Mouse (sf9, His)

The DDC protein plays a key role in neurotransmitter synthesis as it catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to produce dopamine and L-5-hydroxytryptophan to produce serotonin. These enzymatic reactions represent key steps in neurotransmitter biosynthesis and are essential for the normal functioning of the central nervous system. DDC Protein, Mouse (sf9, His) is the recombinant mouse-derived DDC protein, expressed by Sf9 insect cells , with C-His labeled tag. The total length of DDC Protein, Mouse (sf9, His) is 480 a.a., with molecular weight of ~55 kDa.

DDC, or aromatic L-amino acid decarboxylase, is an enzyme that catalyzes the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to produce dopamine and L-5-hydroxytryptophan to serotonin. This enzymatic activity is a critical step in the biosynthesis of neurotransmitters, specifically in the synthesis of dopamine, a neurotransmitter involved in various physiological processes including mood regulation and motor control, and serotonin, which is crucial for mood and emotion regulation. The decarboxylation reactions mediated by DDC are integral to the availability of these neurotransmitters, emphasizing the enzyme's significance in neurochemical pathways. It has to underscore DDC's specific role in the conversion of precursors to neurotransmitters, highlighting its importance in neurotransmitter biosynthesis.

Measured by its ability to convert the substrate 3, 4-dihydroxy L-phenylalanine (L-Dopa) to 3, 4-dihydroxyphenylethylamine (dopamine). The dopamine product is measured by its absorbance at 340 nm after derivatization with trinitrobenzene sulfonic acid. The specific activity is >1500 pmol/min/µg, as measured under the described conditions.

Mouse

Sf9 insect cells

C-His

O88533 (M1-E480)

13195  [NCBI]

Approximately 55 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Solution

Supplied as 0.22 μm filtered solution in 20 mM Tris, 0.3 M NaCl, 10% Glycerol, pH 8.0.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.