1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Isomerases (EC 5)
  4. DDT Protein, Human (His)

DDT Protein, Human (His) is the recombinant human-derived DDT protein, expressed by E. coli, with N-His tag.

For research use only. We do not sell to patients.

Surface Plasmon Resonance (SPR) Assay Service   Protein Expression Service

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  • Biological Activity

  • Technical Parameters

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Description

DDT Protein, Human (His) is the recombinant human-derived DDT protein, expressed by E. coli, with N-His tag.

Background

DDT is a compound involved in the tautomerization of D-dopachrome, which undergoes decarboxylation to produce 5,6-dihydroxyindole (DHI).

Species

Human

Source

E. coli

Tag

N-8*His

Accession

P30046 (P2-L118)

Gene ID

1652

Protein Length

Full Length of Mature Protein

Synonyms
D-dopachrome decarboxylase; D-dopachrome tautomerase; Phenylpyruvate tautomerase II; DDT;
AA Sequence

PFLELDTNLPANRVPAGLEKRLCAAAASILGKPADRVNVTVRPGLAMALSGSTEPCAQLSISSIGVVGTAEDNRSHSAHFFEFLTKELALGQDRILIRFFPLESWQIGKIGTVMTFL

Predicted Molecular Mass
13.9 kDa
Molecular Weight

Approximately 13.9 kDa,based on SDS-PAGE under reducing conditions.

Purity

≥ 95%, as determined by Bis-Tris PAGE.

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50mM Tris, 200mM NaCl, 10% Glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

N/A.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

DDT Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
DDT Protein, Human (His)
Cat. No.:
HY-P703834
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