DDX21 Protein, Human (His)
Based on 1 Customer Validation
DDX21, a member of the DEAD-box subfamily, is a multifunctional RNA helicase that uses energy from ATP hydrolysis to unwind structured RNA molecules, thereby promoting downstream RNA processing or RNA-protein complex remodeling. DDX21 Protein, Human (His) is a recombinant DDX21 protein expressed by *E. coli* with a C-6*His tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- References
- Help & FAQs
Biological Activity
Description
DDX21, a member of the DEAD-box subfamily, is a multifunctional RNA helicase that uses energy from ATP hydrolysis to unwind structured RNA molecules, thereby promoting downstream RNA processing or RNA-protein complex remodeling. DDX21 Protein, Human (His) is a recombinant DDX21 protein expressed by *E. coli* with a C-6*His tag.
Background
DDX21, a member of the DEAD-box subfamily, is a multi-tasking RNA helicase that utilizes energy from ATP hydrolysis to the unwinding of structured RNA molecules, thereby enabling downstream RNA processing or remodeling of RNA-rotein complexes. DDX21 binds to various RNA substrates, such as rRNA, snoRNA, 7SK, and nucleic acid structures, including R-loops that are often formed co-transcriptionally by nascent RNA hybridized with template DNA. DDX21 has been implicated in a wide range of biological processes, including multiple steps in ribosome biogenesis, regulation of RNA polymerase II (RNAPII)-mediated transcription, and sensing of cellular glucose levels for epidermal differentiation, among others. These multifaceted functions have been associated with many developmental defects and diseases, including cancers, possibly reflecting the fact that DDX21 may reside in diverse complexes to mediate corresponding cellular processes[1].
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
Q9NR30-1 (M1-Q783)
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Gene ID9188
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Molecular Construction
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N-term
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DDX21 (M1-Q783)
Accession # Q9NR30-1 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
DDX21; DEAD-Box Helicase 21; DExD-Box Helicase 21; DEAD (Asp-Glu-Ala-Asp) Box Helicase 21; Gu-Alpha; RNA Helicase II/Gu Alpha; Nucleolar RNA Helicase 2; DDX21 Protein; Nucleolar RNA Helicase Gu; RNA Helicase; Nucleolar RNA Helicase II; Gu Protein; DEAD Bo
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AA Sequence
MPGKLRSDAGLESDTAMKKGETLRKQTEEKEKKEKPKSDKTEEIAEEEETVFPKAKQVKKKAEPSEVDMNSPKSKKAKKKEEPSQNDISPKTKSLRKKKEPIEKKVVSSKTKKVTKNEEPSEEEIDAPKPKKMKKEKEMNGETREKSPKLKNGFPHPEPDCNPSEAASEESNSEIEQEIPVEQKEGAFSNFPISEETIKLLKGRGVTFLFPIQAKTFHHVYSGKDLIAQARTGTGKTFSFAIPLIEKLHGELQDRKRGRAPQVLVLAPTRELANQVSKDFSDITKKLSVACFYGGTPYGGQFERMRNGIDILVGTPGRIKDHIQNGKLDLTKLKHVVLDEVDQMLDMGFADQVEEILSVAYKKDSEDNPQTLLFSATCPHWVFNVAKKYMKSTYEQVDLIGKKTQKTAITVEHLAIKCHWTQRAAVIGDVIRVYSGHQGRTIIFCETKKEAQELSQNSAIKQDAQSLHGDIPQKQREITLKGFRNGSFGVLVATNVAARGLDIPEVDLVIQSSPPKDVESYIHRSGRTGRAGRTGVCICFYQHKEEYQLVQVEQKAGIKFKRIGVPSATEIIKASSKDAIRLLDSVPPTAISHFKQSAEKLIEEKGAVEALAAALAHISGATSVDQRSLINSNVGFVTMILQCSIEMPNISYAWKELKEQLGEEIDSKVKGMVFLKGKLGVCFDVPTASVTEIQEKWHDSRRWQLSVATEQPELEGPREGYGGFRGQREGSRGFRGQRDGNRRFRGQREGSRGPRGQRSGGGNKSNRSQNKGQKRSFSKAFGQ
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Predicted Molecular Mass
88.2 kDa
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Molecular Weight
Approximately 93-95 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm filtered solution of PBS, 6% trehalose, pH 7.4.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (265 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
References
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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