DDX58 Protein, Human (His)
Based on 1 Customer Validation
The DDX58 protein is an innate immune receptor that detects cytoplasmic viral nucleic acids and initiates a signaling cascade for type I interferon production.It forms ribonucleoprotein complexes with viral RNA and promotes homo-oligomerization.DDX58 Protein, Human (His) is the recombinant human-derived DDX58 protein, expressed by E.coli , with N-6*His labeled tag.
- Species: Human
- Source: E. coli
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
The DDX58 protein is an innate immune receptor that detects cytoplasmic viral nucleic acids and initiates a signaling cascade for type I interferon production.It forms ribonucleoprotein complexes with viral RNA and promotes homo-oligomerization.DDX58 Protein, Human (His) is the recombinant human-derived DDX58 protein, expressed by E.coli , with N-6*His labeled tag.
DDX58 protein serves as an innate immune receptor crucial for detecting cytoplasmic viral nucleic acids, initiating a downstream signaling cascade that leads to the production of type I interferons and pro-inflammatory cytokines. It forms a ribonucleoprotein complex with viral RNAs, promoting homooligomerization into filaments. This oligomerization facilitates the recruitment of RNF135, an E3 ubiquitin-protein ligase, which amplifies RIG-I-mediated antiviral signaling. Upon activation, DDX58 associates with MAVS/IPS1, activating TBK1 and IKBKE kinases, leading to the phosphorylation of IRF3 and IRF7. This activation prompts the transcription of antiviral immunological genes, including interferons IFN-alpha and IFN-beta. DDX58 recognizes various viral RNA ligands, such as 5'-triphosphorylated ssRNAs and dsRNAs, with the 5'-triphosphate moiety and blunt-end base pairing being crucial for its activity. The protein detects a broad range of RNA viruses and plays a role in antiviral signaling against dsDNA-containing viruses, such as Epstein-Barr virus. Additionally, it participates in granulocyte production, differentiation, bacterial phagocytosis, and the regulation of cell migration.
Technical Parameters
-
Species Human
-
Source E. coli
-
Tag N-6*His
-
Accession
O95786 (M1-L430)
-
Molecular Construction
-
N-term
-
6*His
-
DDX58 (M1-L430)
Accession # O95786 -
C-term
-
-
Protein Length
Partial
-
Synonyms
RIGI; DExD/H-Box Helicase 58; Prev. DDX58; RNA Helicase RIG-I; Antiviral Innate Immune Response Receptor RIG-I; DKFZp434J1111; RIG-I; FLJ13599; Retinoic Acid-Inducible Gene 1 Protein; RLR-1; Retinoic Acid-Inducible Gene I Protein; DEAD/H (Asp-Glu-Ala-Asp/
-
AA Sequence
MTTEQRRSLQAFQDYIRKTLDPTYILSYMAPWFREEEVQYIQAEKNNKGPMEAATLFLKFLLELQEEGWFRGFLDALDHAGYSGLYEAIESWDFKKIEKLEEYRLLLKRLQPEFKTRIIPTDIISDLSECLINQECEEILQICSTKGMMAGAEKLVECLLRSDKENWPKTLKLALEKERNKFSELWIVEKGIKDVETEDLEDKMETSDIQIFYQEDPECQNLSENSCPPSEVSDTNLYSPFKPRNYQLELALPAMKGKNTIICAPTGCGKTFVSLLICEHHLKKFPQGQKGKVVFFANQIPVYEQQKSVFSKYFERHGYRVTGISGATAENVPVEQIVENNDIIILTPQILVNNLKKGTIPSLSIFTLMIFDECHNTSKQHPYNMIMFNYLDQKLGGSSGPLPQVIGLTASVGVGDAKNTDEALDYICKL
-
Predicted Molecular Mass
53.3 kDa
-
Molecular Weight
Approximately 60 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
Lyophilized from a 0.22 μm solution of 10 mM Tris-HCl, 1 mM EDTA, 6% Trehalose, pH 8.0 or PBS, 6% Trehalose, pH 7.4.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
\
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (238 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
