Dit Protein, Bacillus phage SPP1 (FLAG, His)
The Dit protein is an important component that forms a 40 Å wide channel distal to the tail in its homohexameric structure. This unique channel structure plays a crucial role in cellular processes and may facilitate DNA ejection. Dit Protein, Bacillus phage SPP1 (FLAG, His) is the recombinant Dit protein, expressed by E. coli , with N-6*His, N-Flag labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
The Dit protein is an important component that forms a 40 Å wide channel distal to the tail in its homohexameric structure. This unique channel structure plays a crucial role in cellular processes and may facilitate DNA ejection. Dit Protein, Bacillus phage SPP1 (FLAG, His) is the recombinant Dit protein, expressed by E. coli , with N-6*His, N-Flag labeled tag.
The Dit (Distal tail protein) is a homohexameric protein that plays a crucial role in viral DNA ejection. It forms a 40 Angstroms wide channel located at the distal tip of the viral tail. Remarkably, even after the DNA ejection process, Dit remains associated with the tail structure. This implies that the protein's structural features contribute to the stability and functionality of the viral tail. The homohexameric nature of Dit suggests a coordinated and efficient mechanism, possibly aiding in the controlled release of genetic material during the viral life cycle.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His;N-Flag
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Accession
O48459 (N2-V141)
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Gene ID955334
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Molecular Construction
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N-term
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Flag-6*His
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Dit (N2-V141)
Accession # O48459 -
C-term
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Protein Length
Partial
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Synonyms
Distal tail protein; Dit; Gene product 19.1 (Gp19.1)
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AA Sequence
NIYDILDKVFTMMYDGQDLTDYFLVQEVRGRSVYSIEMGKRTIAGVDGGVITTESLPARELEVDAIVFGDGTETDLRRRIEYLNFLLHRDTDVPITFSDEPSRTYYGRYEFATEGDEKGGFHKVTLNFYCQDPLKYGPEV
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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