1. Recombinant Proteins
  2. Enzymes & Regulators
  3. Transferases (EC 2) Lyases (EC 4)
  4. DNA polymerase beta Protein, Human (His)

DNA polymerase beta Protein, Human (His)

Cat. No.: HY-P7883
COA Handling Instructions

DNA polymerase beta Protein, Human (His), a DNA repair enzyme, is involved in various types of DNA repair. DNA polymerase beta Protein performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance.

For research use only. We do not sell to patients.

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10 μg $100 In-stock
50 μg $280 In-stock
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  • Biological Activity

  • Technical Parameters

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  • References

Description

DNA polymerase beta Protein, Human (His), a DNA repair enzyme, is involved in various types of DNA repair. DNA polymerase beta Protein performs base excision repair (BER) required for DNA maintenance, replication, recombination, and drug resistance.

Background

DNA polymerase beta (POLB) belongs to the DNA polymerase type-X family. The structure of DNA polymerase beta has been resolved and shows two domains, an 8-kDa domain consisting of several helices and a 31-kDa domain consisting of several subdomains, the fingers, the thumb, and the palm respectively. It is typically described as a DNA repair enzyme, involved in various types of DNA repair such as Base Excision Repair (BER), Nucleotide Excision Repair (NER), post-replicative Mismatch Repair (MMR), and Double Strand Break Repair (DSBR)[1].

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

E. coli

Tag

C-6*His

Accession

P06746 (S2-E335)

Gene ID
Molecular Construction
N-term
POLB (S2-E335)
Accession # P06746
6*His
C-term
Synonyms
rHuDNA polymerase beta, His; DNA Polymerase Beta; POLB
AA Sequence

SKRKAPQETLNGGITDMLTELANFEKNVSQAIHKYNAYRKAASVIAKYPHKIKSGAEAKKLPGVGTKIAEKIDEFLATGKLRKLEKIRQDDTSSSINFLTRVSGIGPSAARKFVDEGIKTLEDLRKNEDKLNHHQRIGLKYFGDFEKRIPREEMLQMQDIVLNEVKKVDSEYIATVCGSFRRGAESSGDMDVLLTHPSFTSESTKQPKLLHQVVEQLQKVHFITDTLSKGETKFMGVCQLPSKNDEKEYPHRRIDIRLIPKDQYYCGVLYFTGSDIFNKNMRAHALEKGFTINEYTIRPLGVTGVAGEPLPVDSEKDIFDYIQWKYREPKDRSE

Molecular Weight

Approximately 39.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Tris-HCl, 100 mM NaCl, 1 mM DTT, 1 mM EDTA, 50% Glycerol, pH 7.8.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation
References

DNA polymerase beta Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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DNA polymerase beta Protein, Human (His)
Cat. No.:
HY-P7883
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