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  3. Oxidoreductases (EC 1)
  4. Dopamine beta-Hydroxylase Protein, Human (HEK293, His)

Dopamine beta-Hydroxylase Protein, Human (HEK293, His)

Cat. No.: HY-P76874
COA Handling Instructions

Dopamine beta-hydroxylase protein is an essential enzyme involved in the regulation of neurotransmitters. Its main function is to catalyze the hydroxylation of dopamine, converting it into norepinephrine (also known as norepinephrine). Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is the recombinant human-derived Dopamine beta-Hydroxylase protein, expressed by HEK293 , with N-His labeled tag. The total length of Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is 578 a.a., with molecular weight of ~68 kDa.

For research use only. We do not sell to patients.

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  • Biological Activity

  • Technical Parameters

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Description

Dopamine beta-hydroxylase protein is an essential enzyme involved in the regulation of neurotransmitters. Its main function is to catalyze the hydroxylation of dopamine, converting it into norepinephrine (also known as norepinephrine). Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is the recombinant human-derived Dopamine beta-Hydroxylase protein, expressed by HEK293 , with N-His labeled tag. The total length of Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is 578 a.a., with molecular weight of ~68 kDa.

Background

Dopamine beta-Hydroxylase protein is an essential enzyme involved in the regulation of neurotransmitters. Its primary function is to catalyze the hydroxylation of dopamine, converting it into noradrenaline (also known as norepinephrine). This enzymatic activity is crucial for the conversion of dopamine to noradrenaline, playing a significant role in modulating these neurotransmitters within the nervous system. By facilitating this chemical transformation, dopamine beta-Hydroxylase protein contributes to the regulation of neurotransmitter levels and influences various physiological processes in the body.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Human

Source

HEK293

Tag

N-His

Accession

P09172 (S26-G603)

Gene ID
Molecular Construction
N-term
His
DBH (S26-G603)
Accession # P09172
C-term
Synonyms
Dopamine beta-hydroxylase; DBH; DOPBHY
Molecular Weight

Approximately 68 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of PBS, pH 7.4. Normally 5 % - 8 % trehalose, mannitol and 0.01% Tween 80 are added as protectants before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation

Dopamine beta-Hydroxylase Protein, Human (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
Dopamine beta-Hydroxylase Protein, Human (HEK293, His)
Cat. No.:
HY-P76874
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