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Dopamine beta-Hydroxylase Protein, Human (HEK293, His)

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Based on 1 Customer Validation

Dopamine beta-hydroxylase protein is an essential enzyme involved in the regulation of neurotransmitters. Its main function is to catalyze the hydroxylation of dopamine, converting it into norepinephrine (also known as norepinephrine). Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is the recombinant human-derived Dopamine beta-Hydroxylase protein, expressed by HEK293 , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Dopamine beta-Hydroxylase Protein, Human (HEK293, His)
  • Dopamine beta-Hydroxylase Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

Dopamine beta-hydroxylase protein is an essential enzyme involved in the regulation of neurotransmitters. Its main function is to catalyze the hydroxylation of dopamine, converting it into norepinephrine (also known as norepinephrine). Dopamine beta-Hydroxylase Protein, Human (HEK293, His) is the recombinant human-derived Dopamine beta-Hydroxylase protein, expressed by HEK293 , with N-His labeled tag.

Background

Dopamine beta-Hydroxylase protein is an essential enzyme involved in the regulation of neurotransmitters. Its primary function is to catalyze the hydroxylation of dopamine, converting it into noradrenaline (also known as norepinephrine). This enzymatic activity is crucial for the conversion of dopamine to noradrenaline, playing a significant role in modulating these neurotransmitters within the nervous system. By facilitating this chemical transformation, dopamine beta-Hydroxylase protein contributes to the regulation of neurotransmitter levels and influences various physiological processes in the body.

Verified Bioactivity

Measured by its ability to induce Norepinephrine accumulation in SH-SY5Y cells. The ED50 for this effect is 17.76 ng/mL, corresponding to a specific activity is 5.631×104 U/mg.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce Norepinephrine accumulation in SH-SY5Y cells. The ED50 for this effect is 17.76 ng/mL, corresponding to a specific activity is 5.631×104 U/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag N-His
  • Accession

    P09172 (A41-G617)

  • Gene ID
    1621  [NCBI]
  • Molecular Construction
    • N-term
    • His
    • DBH (A41-G617)
      Accession # P09172
    • C-term
  • Protein Length

    Intragranular Domain

  • Synonyms

    DBH; Dopamine Beta-Hydroxylase (Dopamine Beta-Monooxygenase); Dopamine Beta-Hydroxylase; Dopamine Beta-Monooxygenase; DBM; ORTHYP1

  • AA Sequence

    APRESPLPYHIPLDPEGSLELSWNVSYTQEAIHFQLLVRRLKAGVLFGMSDRGELENADLVVLWTDGDTAYFADAWSDQKGQIHLDPQQDYQLLQVQRTPEGLTLLFKRPFGTCDPKDYLIEDGTVHLVYGILEEPFRSLEAINGSGLQMGLQRVQLLKPNIPEPELPSDACTMEVQAPNIQIPSQETTYWCYIKELPKGFSRHHIIKYEPIVTKGNEALVHHMEVFQCAPEMDSVPHFSGPCDSKMKPDRLNYCRHVLAAWALGAKAFYYPEEAGLAFGGPGSSRYLRLEVHYHNPLVIEGRNDSSGIRLYYTAKLRRFNAGIMELGLVYTPVMAIPPRETAFILTGYCTDKCTQLALPPSGIHIFASQLHTHLTGRKVVTVLVRDGREWEIVNQDNHYSPHFQEIRMLKKVVSVHPGDVLITSCTYNTEDRELATVGGFGILEEMCVNYVHYYPQTQLELCKSAVDAGFLQKYFHLINRFNNEDVCTCPQASVSQQFTSVPWNSFNRDVLKALYSFAPISMHCNKSSAVRFQGEWNLQPLPKVISTLEEPTPQCPTSQGRSPAGPTVVSIGGGKG

  • Predicted Molecular Mass

    67.3 kDa

  • Molecular Weight

    Approximately 68 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 90%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose, 5% mannitol and 0.01% Tween 80.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce Norepinephrine accumulation in SH-SY5Y cells. The ED50 for this effect is 17.76 ng/mL, corresponding to a specific activity is 5.631×104 U/mg.

  • Dopamine beta-Hydroxylase Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for Dopamine beta-Hydroxylase Protein, Human (HEK293, His)
  • Dopamine beta-Hydroxylase Protein, Human (HEK293, His) Product Image