DOT1L Protein, Human (Active, sf9, GST)
DOT1L Protein, Human (Active, sf9, GST) is the recombinant human-derived DOT1L protein, expressed by Sf9 insect cells, with N-GST tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
DOT1L Protein, Human (Active, sf9, GST) is the recombinant human-derived DOT1L protein, expressed by Sf9 insect cells, with N-GST tag.
Background
KMT4 is a histone methyltransferase that methylates histone H3 at Lys-79. It shows a preference for nucleosomes as substrates over free histones (by similar: PubMed:12123582). Additionally, KMT4 has DNA-binding activity (by similar: PubMed:12628190).
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag N-GST
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Accession
Q8TEK3-2 (M1-A435)
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Gene ID84444
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Molecular Construction
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N-term
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GST
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DOT1L (M1-A435)
Accession # Q8TEK3-2 -
C-term
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Protein Length
Partial
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Synonyms
DOT1L; Lysine N-Methyltransferase 4; DOT1 Like Histone Lysine Methyltransferase; DOT1-Like Protein; KMT4; H3-K79-HMTase; Histone-Lysine N-Methyltransferase, H3 Lysine-79 Specific; DOT1-Like, Histone H3 Methyltransferase (S. Cerevisiae); Histone H3-K79 Met
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AA Sequence
MGEKLELRLKSPVGAEPAVYPWPLPVYDKHHDAAHEIIETIRWVCEEIPDLKLAMENYVLIDYDTKSFESMQRLCDKYNRAIDSIHQLWKGTTQPMKLNTRPSTGLLRHILQQVYNHSVTDPEKLNNYEPFSPEVYGETSFDLVAQMIDEIKMTDDDLFVDLGSGVGQVVLQVAAATNCKHHYGVEKADIPAKYAETMDREFRKWMKWYGKKHAEYTLERGDFLSEEWRERIANTSVIFVNNFAFGPEVDHQLKERFANMKEGGRIVSSKPFAPLNFRINSRNLSDIGTIMRVVELSPLKGSVSWTGKPVSYYLHTIDRTILENYFSSLKNPKLREEQEAARRRQQRESKSNAATPTKGPEGKVAGPADAPMDSGAEEEKAGAATVKKPSPSKARKKKLNKKGRKMAGRKRGRPKKMNTANPERKPKKNQTALDA
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Predicted Molecular Mass
78 kDa
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Molecular Weight
Approximately 78 kDa, based on SDS-PAGE under reducing conditions.
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Structure/Form
Monomer
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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