DPYSL3 Protein, Human (His)
Based on 1 Customer Validation
The DPYSL3 protein plays a key role in class 3 semaphorin-triggered signaling, driving cytoskeletal remodeling that is critical for axon guidance, growth cone collapse, and cell migration. As a component of homo- and heterotetramers of CRMP1, DPYSL2, DPYSL4 or DPYSL5, it is intricately associated with related proteins, highlighting its involvement in complex cellular dynamics. DPYSL3 Protein, Human (His) is the recombinant human-derived DPYSL3 protein, expressed by E. coli , with N-His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The DPYSL3 protein plays a key role in class 3 semaphorin-triggered signaling, driving cytoskeletal remodeling that is critical for axon guidance, growth cone collapse, and cell migration. As a component of homo- and heterotetramers of CRMP1, DPYSL2, DPYSL4 or DPYSL5, it is intricately associated with related proteins, highlighting its involvement in complex cellular dynamics. DPYSL3 Protein, Human (His) is the recombinant human-derived DPYSL3 protein, expressed by E. coli , with N-His labeled tag.
Background
DPYSL3 protein is indispensable for the signaling cascades initiated by class 3 semaphorins and the subsequent remodeling of the cytoskeleton. It actively contributes to axon guidance, neuronal growth cone collapse, and cell migration, underlining its multifaceted role in cellular processes. As part of homotetramers and heterotetramers with CRMP1, DPYSL2, DPYSL4, or DPYSL5, it forms intricate associations with related proteins, reflecting its involvement in complex cellular dynamics. Additionally, DPYSL3 interacts with synaptic vesicle protein 2 and the SH3A domain of intersectin, further highlighting its participation in molecular interactions crucial for cellular functions. Notably, DPYSL3 engages with FLNA, as demonstrated in its interaction with FLNA's calponin-homology (CH) domain 1 and filamin repeat 24, emphasizing its role in cytoskeletal rearrangements mediated by FLNA.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q14195 (M1-S570)
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Molecular Construction
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N-term
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His
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DPYSL3 (M1-S570)
Accession # Q14195 -
C-term
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Protein Length
Full Length
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Synonyms
DPYSL3; CRMP-4; Dihydropyrimidinase Like 3; ULIP-1; DRP-3; DRP3; CRMP4; Collapsin Response Mediator Protein 4 Long; ULIP; Collapsin Response Mediator Protein 4; Dihydropyrimidinase-Related Protein 3; DPYSL3 Protein; Testicular Secretory Protein Li 7; LCRM
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AA Sequence
MSYQGKKNIPRITSDRLLIKGGRIVNDDQSFYADIYMEDGLIKQIGDNLIVPGGVKTIEANGKMVIPGGIDVHTHFQMPYKGMTTVDDFFQGTKAALAGGTTMIIDHVVPEPESSLTEAYEKWREWADGKSCCDYALHVDITHWNDSVKQEVQNLIKDKGVNSFMVYMAYKDLYQVSNTELYEIFTCLGELGAIAQVHAENGDIIAQEQTRMLEMGITGPEGHVLSRPEELEAEAVFRAITIASQTNCPLYVTKVMSKSAADLISQARKKGNVVFGEPITASLGIDGTHYWSKNWAKAAAFVTSPPLSPDPTTPDYINSLLASGDLQLSGSAHCTFSTAQKAIGKDNFTAIPEGTNGVEERMSVIWDKAVATGKMDENQFVAVTSTNAAKIFNLYPRKGRISVGSDSDLVIWDPDAVKIVSAKNHQSAAEYNIFEGMELRGAPLVVICQGKIMLEDGNLHVTQGAGRFIPCSPFSDYVYKRIKARRKMADLHAVPRGMYDGPVFDLTTTPKGGTPAGSARGSPTRPNPPVRNLHQSGFSLSGTQVDEGVRSASKRIVAPPGGRSNITSLS
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Predicted Molecular Mass
66 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from a 0.22 μm filtered solution of 20 mM Tris-HCl, 0.5 M NaCl, 6% trehalose, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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