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  3. DRD3 Protein, Human (sf9, Flag, His)

DRD3 Protein, Human (sf9, Flag, His) is the recombinant human-derived DRD3, expressed by Sf9 insect cells , with His, Flag labeled tag. ,

For research use only. We do not sell to patients.

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  • Biological Activity

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Description

DRD3 Protein, Human (sf9, Flag, His) is the recombinant human-derived DRD3, expressed by Sf9 insect cells , with His, Flag labeled tag. ,

Background

DRD3 is a dopamine receptor whose activity is mediated by G proteins that inhibit adenylyl cyclase. It promotes cell proliferation.

Species

Human

Source

Sf9 insect cells

Tag

C-His;N-Flag

Accession

P35462-1 (M1-K221,G319-C400, L119W)

Gene ID

1814

Protein Length

Partial

Synonyms
D(3) dopamine receptor; Dopamine D3 receptor; DRD3; Homo sapiens; Human; G-protein coupled receptor; Receptor; Transducer
AA Sequence

GVPLREKKATQMVAIVLGAFIVCWLPFFLTHVLNTHCQTCHVSPELYSATTWLGYVNSALNPVIYTTFNIEFRKAFLKILSC

Molecular Weight

Approximately 54.8 kDa

Glycosylation
Yes
Purity

≥ 85%, as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM HEPES , pH 7.5, 150 mM NaCl, 0.01% (w/v) LMNG, 0.001% (w/v) CHS.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

Please use rapid thawing with running water to thaw the protein.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

DRD3 Protein, Human (sf9, Flag, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
DRD3 Protein, Human (sf9, Flag, His)
Cat. No.:
HY-P703117
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