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DSG3 Protein, Mouse (HEK293, His)

Customer Review

Based on 1 Customer Validation

DSG3 protein facilitates cell-cell adhesion by mediating the interaction between plaque proteins and intermediate filaments at intercellular desmosome junctions. It interacts with PKP2, enhancing the stability and integrity of these junctions. DSG3 plays a vital role in maintaining tissue architecture and structural integrity through strong cell adhesion. DSG3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DSG3 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for DSG3 Protein, Mouse (HEK293, His)
  • DSG3 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

DSG3 protein facilitates cell-cell adhesion by mediating the interaction between plaque proteins and intermediate filaments at intercellular desmosome junctions. It interacts with PKP2, enhancing the stability and integrity of these junctions. DSG3 plays a vital role in maintaining tissue architecture and structural integrity through strong cell adhesion. DSG3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived DSG3 protein, expressed by HEK293 , with C-His labeled tag.

Background

DSG3 protein is a vital component of intercellular desmosome junctions, playing a crucial role in facilitating cell-cell adhesion by mediating the interaction between plaque proteins and intermediate filaments. Notably, DSG3 interacts with PKP2, further contributing to the stability and integrity of these junctions. This protein is essential for maintaining the structural integrity of tissues by promoting strong cell adhesion and proper tissue architecture.

Verified Bioactivity

Measured by the ability of immobilized DSG3 to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 1.343 μg/mL, corresponding to a specific activity is 744.602 units/mg.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by the ability of immobilized DSG3 to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 1.343 μg/mL, corresponding to a specific activity is 744.602 units/mg.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-8*His
  • Accession

    O35902-1 (E50-P617)

  • Gene ID
    13512  [NCBI]
  • Molecular Construction
    • N-term
    • DSG3 (E50-P617)
      Accession # O35902-1
    • 8*His
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    DSG3; Desmoglein-3; Desmoglein 3; PVA; CDHF6; Desmoglein 3 (Pemphigus Vulgaris Antigen); 130 KDa Pemphigus Vulgaris Antigen; Pemphigus Vulgaris Antigen; Cadherin Family Member 6; ABOLM

  • AA Sequence

    EWVKFAKPCREREDNSRRNPIAKITSDFQKNQKITYRISGVGIDQPPFGIFVVDPNNGDINITAIVDREETPSFLITCRALNALGQDVERPLILTVKILDVNDNPPIFSQTIFKGEIEENSASNSLVMILNATDADEPNHMNSKIAFKIVSQEPAGMSMFLISRNTGEVRTLTSSLDREQISSYHLVVSGADNDGTGLSTQCECSIKIKDVNDNFPVLRESQYSARIEENTLNAELLRFQVTDWDEEYTDNWLAVYFFTSGNEGNWFEIETDPRTNEGILKVVKALDYEQVQSMQFSIAVRNKAEFHQSVISQYRVQSTPVTIQVIDVREGISFRPPSKTFTVQRGVSTNKLVGYILGTYQATDEDTGKAASSVRYVLGRNDGGLLVIDSKTAQIKFVKNIDRDSTFIVNKTISAEVLAIDENTGKTSTGTIYVEVPSFNENCPSVVLEKKDICTSSPSVTLSVRTLDRGKYTGPYTVSLEEQPLKLPVMWTITTLNATSALLQAQQQVSPGVYNVPVIVKDNQDGLCDTPESLTLTVCQCDDRSMCRAPIPSREPNTYGESSWRLGP

  • Predicted Molecular Mass

    64.3 kDa

  • Molecular Weight

    Approximately 70-75 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4. Normally 8% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
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    MedChemExpress Validation
     Measured by the ability of immobilized DSG3 to support the adhesion of Caki-2 human clear cell carcinoma epithelial cells. The ED50 for this effect is 1.343 μg/mL, corresponding to a specific activity is 744.602 units/mg.

  • DSG3 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for DSG3 Protein, Mouse (HEK293, His)
  • DSG3 Protein, Mouse (HEK293, His) Product Image