DST Protein, Human (P.pastoris, His)
DST proteins integrate intermediate filaments, actin, and microtubule networks. DST Protein, Human (P.pastoris, His) is the recombinant human-derived DST protein, expressed by P. pastoris , with N-His labeled tag.
- Species: Human
- Source: P. pastoris
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
DST proteins integrate intermediate filaments, actin, and microtubule networks. DST Protein, Human (P.pastoris, His) is the recombinant human-derived DST protein, expressed by P. pastoris , with N-His labeled tag.
Background
DST Protein serves as a crucial cytoskeletal linker, integrating intermediate filaments, actin, and microtubule cytoskeleton networks. Its role varies across cell types, anchoring intermediate filaments to the actin cytoskeleton in neural and muscle cells or connecting keratin-containing intermediate filaments to hemidesmosomes in epithelial cells. DST Protein may self-aggregate to form filaments or a two-dimensional mesh. In sensory neurons, it regulates the organization and stability of the microtubule network, facilitating axonal transport. Additionally, it mediates the docking of the dynein/dynactin motor complex to vesicle cargos for retrograde axonal transport through interactions with TMEM108 and DCTN1. In epithelial cells, DST Protein plays a structural role in assembling hemidesmosomes, anchoring keratin-containing intermediate filaments to the inner plaque and contributing to the regulation of keratinocyte polarity and motility, particularly in mediating integrin ITGB4's control of RAC1 activity.
Technical Parameters
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Species Human
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Source P. pastoris
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Tag N-His
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Accession
Q03001-3 (M1-G195)
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Molecular Construction
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N-term
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His
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DST (M1-G195)
Accession # Q03001-3 -
C-term
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Protein Length
Partial
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Synonyms
DST; DMH; Prev. BPAG1; Bullous Pemphigoid Antigen 1, 230/240kDa; BP240; 230/240 KDa Bullous Pemphigoid Antigen; BPA; 230 KDa Bullous Pemphigoid Antigen; Hemidesmosomal Plaque Protein; Alternative Protein DST; Bullous Pemphigoid Antigen 1; Trabeculin-Beta;
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AA Sequence
MHSSSYSYRSSDSVFSNTTSTRTSLDSNENLLLVHCGPTLINSCISFGSESFDGHRLEMLQQIANRVQRDSVICEDKLILAGNALQSDSKRLESGVQFQNEAEIAGYILECENLLRQHVIDVQILIDGKYYQADQLVQRVAKLRDEIMALRNECSSVYSKGRILTTEQTKLMISGITQSLNSGFAQTLHPSLTSG
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Predicted Molecular Mass
23.7 kDa
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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