EMAP-II Protein, Human (His)
EMAP-II Protein, Human (His) is the recombinant human-derived EMAP-II protein, expressed by E. coli, with N-His tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
EMAP-II Protein, Human (His) is the recombinant human-derived EMAP-II protein, expressed by E. coli, with N-His tag.
Background
EMAP-II serves as a non-catalytic component within the multisynthase complex (MSC), playing a crucial role in orchestrating the functions of various tRNA ligases. It specifically stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase and binds to tRNA. Apart from its role in protein synthesis, EMAP-II exhibits inflammatory cytokine activity and negatively regulates TGF-beta signaling by stabilizing SMURF2 and inhibiting its SMAD7-mediated degradation. Additionally, EMAP-II contributes to glucose homeostasis through the induction of glucagon secretion, promotes dermal fibroblast proliferation and wound repair, and modulates endothelial cell responses by inducing migration at low concentrations and apoptosis at high concentrations. It forms homodimers and is part of the MSC, interacting with various tRNA ligases, SMURF2, HSP90B1/gp96, and PSMA7, showcasing its involvement in diverse cellular processes and regulatory networks.
Technical Parameters
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Species Human
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Source E. coli
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Tag N-His
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Accession
Q12904-1 (A2-K312)
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Gene ID9255
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Molecular Construction
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N-term
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His
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EMAP-II (A2-K312)
Accession # Q12904-1 -
C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
AIMP1; Multisynthase Complex Auxiliary Component P43; Prev. SCYE1; EMAP-2; EMAPII; EMAP2; P43; Endothelial-Monocyte Activating Polypeptide II; Small Inducible Cytokine Subfamily E, Member 1 (Endothelial Monocyte-Activating); Endothelial Monocyte-Activatin
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AA Sequence
ANNDAVLKRLEQKGAEADQIIEYLKQQVSLLKEKAILQATLREEKKLRVENAKLKKEIEELKQELIQAEIQNGVKQIPFPSGTPLHANSMVSENVIQSTAVTTVSSGTKEQIKGGTGDEKKAKEKIEKKGEKKEKKQQSIAGSADSKPIDVSRLDLRIGCIITARKHPDADSLYVEEVDVGEIAPRTVVSGLVNHVPLEQMQNRMVILLCNLKPAKMRGVLSQAMVMCASSPEKIEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNDECVATYKGVPFEVKGKGVCRAQTMSNSGIK
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Predicted Molecular Mass
35.4 kDa
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Molecular Weight
Approximately 40-45 kDa, based on SDS-PAGE under reducing conditions.
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Structure/Form
Monomer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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