EMAP-II Protein, Mouse (His)

EMAP-II is a noncatalytic component of the multisynthase complex (MSC) and plays a critical role in coordinating tRNA ligase function. It stimulates cytoplasmic arginyl-tRNA synthase and binds to tRNA. EMAP-II Protein, Mouse (His) is the recombinant mouse-derived EMAP-II protein, expressed by E. coli , with N-His labeled tag.

EMAP-II serves as a non-catalytic component within the multisynthase complex (MSC), playing a crucial role in orchestrating the functions of various tRNA ligases. It specifically stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase and binds to tRNA. Apart from its role in protein synthesis, EMAP-II exhibits inflammatory cytokine activity and negatively regulates TGF-beta signaling by stabilizing SMURF2 and inhibiting its SMAD7-mediated degradation. Additionally, EMAP-II contributes to glucose homeostasis through the induction of glucagon secretion, promotes dermal fibroblast proliferation and wound repair, and modulates endothelial cell responses by inducing migration at low concentrations and apoptosis at high concentrations. It forms homodimers and is part of the MSC, interacting with various tRNA ligases, SMURF2, HSP90B1/gp96, and PSMA7, showcasing its involvement in diverse cellular processes and regulatory networks.

Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. When rmEMAP-II is coated, it will induce TNF-alpha production by RAW 264.7 cells in the presence of Polymyxin B (20 µg/mL) with an ED₅₀ is 5.179 µg/mL.

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  Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. When rmEMAP-II is coated, it will induce TNF-alpha production by RAW 264.7 cells in the presence of Polymyxin B (20 µg/mL) with an ED₅₀ is 5.179 µg/mL, corresponding to a specific activity is 193.0875 U/mg.

Mouse

E. coli

N-8*His

P31230 (A2-K310)

13722

Full Length of Mature Protein

ATNDAVLKRLEQKGAEADQIIEYLKQQVALLKEKAILQATMREEKKLRVENAKLKKEIEELKQELILAEIHNGVEQVRVRLSTPLQTNCTASESVVQSPSVATTASPATKEQIKAGEEKKVKEKTEKKGEKKEKQQSAAASTDSKPIDASRLDLRIGCIVTAKKHPDADSLYVEEVDVGEAAPRTVVSGLVNHVPLEQMQNRMVVLLCNLKPAKMRGVLSQAMVMCASSPEKVEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNAECVATYKGAPFEVKGKGVCRAQTMANSGIK

Approximately 42-46 kDa, based on SDS-PAGE under reducing conditions.

≥ 95%, as determined by Bis-Tris PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.

• [1]. /biology-dictionary/mcf7-cell.html  [Content Brief]