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EMAP-II Protein, Mouse (His)

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Based on 1 Customer Validation

EMAP-II is a noncatalytic component of the multisynthase complex (MSC) and plays a critical role in coordinating tRNA ligase function. It stimulates cytoplasmic arginyl-tRNA synthase and binds to tRNA. EMAP-II Protein, Mouse (His) is the recombinant mouse-derived EMAP-II protein, expressed by E. coli , with N-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: E. coli
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for EMAP-II Protein, Mouse (His)
  • EMAP-II Protein, Mouse (His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

EMAP-II is a noncatalytic component of the multisynthase complex (MSC) and plays a critical role in coordinating tRNA ligase function. It stimulates cytoplasmic arginyl-tRNA synthase and binds to tRNA. EMAP-II Protein, Mouse (His) is the recombinant mouse-derived EMAP-II protein, expressed by E. coli , with N-His labeled tag.

Background

EMAP-II serves as a non-catalytic component within the multisynthase complex (MSC), playing a crucial role in orchestrating the functions of various tRNA ligases. It specifically stimulates the catalytic activity of cytoplasmic arginyl-tRNA synthase and binds to tRNA. Apart from its role in protein synthesis, EMAP-II exhibits inflammatory cytokine activity and negatively regulates TGF-beta signaling by stabilizing SMURF2 and inhibiting its SMAD7-mediated degradation. Additionally, EMAP-II contributes to glucose homeostasis through the induction of glucagon secretion, promotes dermal fibroblast proliferation and wound repair, and modulates endothelial cell responses by inducing migration at low concentrations and apoptosis at high concentrations. It forms homodimers and is part of the MSC, interacting with various tRNA ligases, SMURF2, HSP90B1/gp96, and PSMA7, showcasing its involvement in diverse cellular processes and regulatory networks.

Verified Bioactivity

Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. When rmEMAP-II is coated, it will induce TNF-alpha production by RAW 264.7 cells in the presence of Polymyxin B (20 µg/mL) with an ED50 is 5.179 µg/mL.

MCE Validation Data

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. When rmEMAP-II is coated, it will induce TNF-alpha production by RAW 264.7 cells in the presence of Polymyxin B (20 µg/mL) with an ED50 is 5.179 µg/mL, corresponding to a specific activity is 193.0875 U/mg.

Technical Parameters

  • Species Mouse
  • Source E. coli
  • Tag N-8*His
  • Accession

    P31230 (A2-K310)

  • Gene ID
    13722
  • Molecular Construction
    • N-term
    • His
    • EMAP-II (A2-K310)
      Accession # P31230
    • C-term
  • Protein Length

    Full Length of Mature Protein

  • Synonyms

    AIMP1; Multisynthase Complex Auxiliary Component P43; Prev. SCYE1; EMAP-2; EMAPII; EMAP2; P43; Endothelial-Monocyte Activating Polypeptide II; Small Inducible Cytokine Subfamily E, Member 1 (Endothelial Monocyte-Activating); Endothelial Monocyte-Activatin

  • AA Sequence

    ATNDAVLKRLEQKGAEADQIIEYLKQQVALLKEKAILQATMREEKKLRVENAKLKKEIEELKQELILAEIHNGVEQVRVRLSTPLQTNCTASESVVQSPSVATTASPATKEQIKAGEEKKVKEKTEKKGEKKEKQQSAAASTDSKPIDASRLDLRIGCIVTAKKHPDADSLYVEEVDVGEAAPRTVVSGLVNHVPLEQMQNRMVVLLCNLKPAKMRGVLSQAMVMCASSPEKVEILAPPNGSVPGDRITFDAFPGEPDKELNPKKKIWEQIQPDLHTNAECVATYKGAPFEVKGKGVCRAQTMANSGIK

  • Predicted Molecular Mass

    35.1 kDa

  • Molecular Weight

    Approximately 42-46 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by Bis-Tris PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 20 mM Tris, 500 mM NaCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     Measured by its ability to induce TNF-alpha secretion by RAW 264.7 mouse monocyte/macrophage cells. When rmEMAP-II is coated, it will induce TNF-alpha production by RAW 264.7 cells in the presence of Polymyxin B (20 µg/mL) with an ED50 is 5.179 µg/mL, corresponding to a specific activity is 193.0875 U/mg.

  • EMAP-II Protein, Mouse (His) Product Image
    1/16
  • Experimental Validation Results for EMAP-II Protein, Mouse (His)
  • EMAP-II Protein, Mouse (His) Product Image