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EphB2 Protein, Mouse (HEK293, Fc)

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EphB2 protein is a member of the Eph receptor family and has a ligand-binding domain, a transmembrane region, and an intracellular kinase domain. It preferentially binds ephrin-B ligands and plays a role in nervous system and vascular development. EphB2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived EphB2 protein, expressed by HEK293 , with C-hFc labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for EphB2 Protein, Mouse (HEK293, Fc)
  • Experimental Validation Results for EphB2 Protein, Mouse (HEK293, Fc)
  • EphB2 Protein, Mouse (HEK293, Fc) Product Image
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609-228-6898
[email protected] [email protected]
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  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

EphB2 protein is a member of the Eph receptor family and has a ligand-binding domain, a transmembrane region, and an intracellular kinase domain. It preferentially binds ephrin-B ligands and plays a role in nervous system and vascular development. EphB2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived EphB2 protein, expressed by HEK293 , with C-hFc labeled tag.

Background

EphB2, a member of the Eph receptor family, encodes a transmembrane glycoprotein with a ligand-binding domain, transmembrane region, and intracellular kinase domain. This receptor exhibits a preference for binding membrane-bound ephrin-B ligands, contributing to its involvement in nervous system and vascular development. Additionally, EphB2 serves as a marker for intestinal stem cells. Homozygous knockout mice for this gene demonstrate impaired axon guidance and vestibular function. The broad expression of EphB2 across various tissues, including the developing brain and central nervous system, underscores its crucial role in mediating diverse cellular processes during development.

Verified Bioactivity

1.This product does not contain protein kinase domain.
2.Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB2 at 10µg/mL (100 µL/well) can bind Biotinylated Mouse Ephrin-B2 protein. The ED50 for this effect is 4.958 ng/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB2 at 10µg/mL (100 µL/well) can bind Biotinylated Mouse Ephrin-B2 protein. The ED50 for this effect is 4.958 ng/mL.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag C-hFc
  • Accession

    P54763-3/NP_034272.1 (V19-K540)

  • Gene ID
    13844  [NCBI]
  • Molecular Construction
    • N-term
    • EphB2 (V19-K540)
      Accession # P54763-3/NP_034272.1
    • hFc
    • C-term
  • Protein Length

    Extracellular Domain

  • Synonyms

    EPHB2; Ephrin Type-B Receptor 2 Variant; Prev. EPHT3; Protein-Tyrosine Kinase HEK5; Prev. DRT; Elk-Related Tyrosine Kinase; Prev. ERK; ELK-Related Tyrosine Kinase; Tyrosine-Protein Kinase Receptor EPH-3; Eph Tyrosine Kinase 3; Ephrin Type-B Receptor 2; EP

  • AA Sequence

    VEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEK

  • Molecular Weight

    Approximately 97 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB2 at 10µg/mL (100 µL/well) can bind Biotinylated Mouse Ephrin-B2 protein. The ED50 for this effect is 4.958 ng/mL.

  • EphB2 Protein, Mouse (HEK293, Fc) Product Image
    1/16
  • Experimental Validation Results for EphB2 Protein, Mouse (HEK293, Fc)
  • Experimental Validation Results for EphB2 Protein, Mouse (HEK293, Fc)
  • EphB2 Protein, Mouse (HEK293, Fc) Product Image