EphB2 Protein, Mouse (HEK293, Fc)

EphB2 protein is a member of the Eph receptor family and has a ligand-binding domain, a transmembrane region, and an intracellular kinase domain. It preferentially binds ephrin-B ligands and plays a role in nervous system and vascular development. EphB2 Protein, Mouse (HEK293, Fc) is the recombinant mouse-derived EphB2 protein, expressed by HEK293 , with C-hFc labeled tag. The total length of EphB2 Protein, Mouse (HEK293, Fc) is 522 a.a., with molecular weight of ~96.6 kDa.

EphB2, a member of the Eph receptor family, encodes a transmembrane glycoprotein with a ligand-binding domain, transmembrane region, and intracellular kinase domain. This receptor exhibits a preference for binding membrane-bound ephrin-B ligands, contributing to its involvement in nervous system and vascular development. Additionally, EphB2 serves as a marker for intestinal stem cells. Homozygous knockout mice for this gene demonstrate impaired axon guidance and vestibular function. The broad expression of EphB2 across various tissues, including the developing brain and central nervous system, underscores its crucial role in mediating diverse cellular processes during development.

Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB2 at 10µg/mL (100 µL/well) can bind Biotinylated Mouse Ephrin-B2 protein. The ED50 for this effect is 4.958 ng/mL.

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  "Measured by its binding ability in a functional ELISA. Immobilized Mouse EphB2 at 10µg/mL (100 µL/well) can bind Biotinylated Mouse Ephrin-B2 protein. The ED₅₀ for this effect is 4.958 ng/mL. "

Mouse

HEK293

C-hFc

NP_034272.1 (V19-K540)

13844  [NCBI]

VEETLMDSTTATAELGWMVHPPSGWEEVSGYDENMNTIRTYQVCNVFESSQNNWLRTKFIRRRGAHRIHVEMKFSVRDCSSIPSVPGSCKETFNLYYYEADFDLATKTFPNWMENPWVKVDTIAADESFSQVDLGGRVMKINTEVRSFGPVSRNGFYLAFQDYGGCMSLIAVRVFYRKCPRIIQNGAIFQETLSGAESTSLVAARGSCIANAEEVDVPIKLYCNGDGEWLVPIGRCMCKAGFEAVENGTVCRGCPSGTFKANQGDEACTHCPINSRTTSEGATNCVCRNGYYRADLDPLDMPCTTIPSAPQAVISSVNETSLMLEWTPPRDSGGREDLVYNIICKSCGSGRGACTRCGDNVQYAPRQLGLTEPRIYISDLLAHTQYTFEIQAVNGVTDQSPFSPQFASVNITTNQAAPSAVSIMHQVSRTVDSITLSWSQPDQPNGVILDYELQYYEKELSEYNATAIKSPTNTVTVQGLKAGAIYVFQVRARTVAGYGRYSGKMYFQTMTEAEYQTSIKEK

Approximately 96.6 kDa

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  Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.