ERAP2 Protein, Human (HEK293, His)
Based on 1 Customer Validation
ERAP2 protein, a key aminopeptidase, plays a pivotal role in peptide trimming, crucial for generating peptides binding to HLA class I molecules. By selectively hydrolyzing basic residues like Arginine (Arg) and Lysine (Lys), ERAP2 precisely customizes longer precursor peptides, ensuring optimal length for effective presentation on MHC class I molecules. ERAP2 Protein, Human (HEK293, His) is the recombinant human-derived ERAP2 protein, expressed by HEK293 , with N-10*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
ERAP2 protein, a key aminopeptidase, plays a pivotal role in peptide trimming, crucial for generating peptides binding to HLA class I molecules. By selectively hydrolyzing basic residues like Arginine (Arg) and Lysine (Lys), ERAP2 precisely customizes longer precursor peptides, ensuring optimal length for effective presentation on MHC class I molecules. ERAP2 Protein, Human (HEK293, His) is the recombinant human-derived ERAP2 protein, expressed by HEK293 , with N-10*His labeled tag.
Background
ERAP2 protein serves as a key aminopeptidase, playing a pivotal role in the crucial process of peptide trimming. This step is essential for the generation of the majority of peptides that bind to HLA class I molecules. By selectively hydrolyzing basic residues such as Arginine (Arg) and Lysine (Lys), ERAP2 contributes to the precise customization of longer precursor peptides, ensuring they attain the appropriate length for effective presentation on MHC class I molecules.
Verified Bioactivity
Measured by its ability to cleave 50μM fluorogenic peptide substrate, Arg-7-amido-4-methylcoumarin (Arg-AMC) that incubate at room temperature in kinetic mode for 5 minutes. The specific activity is 500.41 pmol/min/μg.
Technical Parameters
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Species Human
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Source HEK293
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Tag N-10*His
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Accession
Q6P179-1/NP_071745.1 (A56-T960)
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Molecular Construction
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N-term
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10*His
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ERAP2 (A56-T960)
Accession # Q6P179-1/NP_071745.1 -
C-term
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Protein Length
Partial
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Synonyms
ERAP2; LRAP; Endoplasmic Reticulum Aminopeptidase 2; Leukocyte-Derived Arginine Aminopeptidase; L-RAP; Aminopeptidase
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AA Sequence
ATNGERFPWQELRLPSVVIPLHYDLFVHPNLTSLDFVASEKIEVLVSNATQFIILHSKDLEITNATLQSEEDSRYMKPGKELKVLSYPAHEQIALLVPEKLTPHLKYYVAMDFQAKLGDGFEGFYKSTYRTLGGETRILAVTDFEPTQARMAFPCFDEPLFKANFSIKIRRESRHIALSNMPKVKTIELEGGLLEDHFETTVKMSTYLVAYIVCDFHSLSGFTSSGVKVSIYASPDKRNQTHYALQASLKLLDFYEKYFDIYYPLSKLDLIAIPDFAPGAMENWGLITYRETSLLFDPKTSSASDKLWVTRVIAHELAHQWFGNLVTMEWWNDIWLKEGFAKYMELIAVNATYPELQFDDYFLNVCFEVITKDSLNSSRPISKPAETPTQIQEMFDEVSYNKGACILNMLKDFLGEEKFQKGIIQYLKKFSYRNAKNDDLWSSLSNSCLESDFTSGGVCHSDPKMTSNMLAFLGENAEVKEMMTTWTLQKGIPLLVVKQDGCSLRLQQERFLQGVFQEDPEWRALQERYLWHIPLTYSTSSSNVIHRHILKSKTDTLDLPEKTSWVKFNVDSNGYYIVHYEGHGWDQLITQLNQNHTLLRPKDRVGLIHDVFQLVGAGRLTLDKALDMTYYLQHETSSPALLEGLSYLESFYHMMDRRNISDISENLKRYLLQYFKPVIDRQSWSDKGSVWDRMLRSALLKLACDLNHAPCIQKAAELFSQWMESSGKLNIPTDVLKIVYSVGAQTTAGWNYLLEQYELSMSSAEQNKILYALSTSKHQEKLLKLIELGMEGKVIKTQNLAALLHAIARRPKGQQLAWDFVRENWTHLLKKFDLGSYDIRMIISGTTAHFSSKDKLQEVKLFFESLEAQGSHLDIFQTVLETITKNIKWLEKNLPTLRTWLMVNT
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Molecular Weight
Approximately 115-125 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 12.5 mM Tris-HCl, 75 mM NaCl, pH 7.5, 50% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (241 KB)
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SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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