ERP27 Protein, Human (HEK293, His)
The ERP27 protein has a unique role in that it can specifically bind unfolded proteins and recruit the protein disulfide isomerase PDIA3 to the substrate through the hydrophobic pocket in its C-terminal domain. This interaction implies that ERP27 is involved in the unfolding stress response and resolves challenges in cellular proteostasis. ERP27 Protein, Human (HEK293, His) is the recombinant human-derived ERP27 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The ERP27 protein has a unique role in that it can specifically bind unfolded proteins and recruit the protein disulfide isomerase PDIA3 to the substrate through the hydrophobic pocket in its C-terminal domain. This interaction implies that ERP27 is involved in the unfolding stress response and resolves challenges in cellular proteostasis. ERP27 Protein, Human (HEK293, His) is the recombinant human-derived ERP27 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
ERP27, a protein with a distinct role, exhibits specific binding to unfolded proteins and possesses the potential to recruit protein disulfide isomerase PDIA3 to unfolded substrates. This binding to protein substrates is facilitated through a hydrophobic pocket located in the C-terminal domain of ERP27. The protein's interaction with unfolded substrates suggests its potential involvement in the unfolded stress response, implying a role in cellular mechanisms addressing proteostasis challenges. Additionally, ERP27 engages in interactions with PDIA3, emphasizing its association with the intricate network of cellular proteins involved in protein folding and quality control. The specific molecular interactions and binding properties of ERP27 underscore its potential significance in cellular stress responses and protein homeostasis pathways.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
Q96DN0 (E26-L273)
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Molecular Construction
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N-term
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ERP27 (E26-L273)
Accession # Q96DN0 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
ERP27; Inactive Protein Disulfide-Isomerase 27; Prev. C12orf46; ER Protein 27; PDIA8; FLJ32115; Protein Disulfide Isomerase Family A, Member 8; Chromosome 12 Open Reading Frame 46; Endoplasmic Reticulum Resident Protein 27; ERp27; Endoplasmic Reticulum Pr
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AA Sequence
EVEKSSDGPGAAQEPTWLTDVPAAMEFIAATEVAVIGFFQDLEIPAVPILHSMVQKFPGVSFGISTDSEVLTHYNITGNTICLFRLVDNEQLNLEDEDIESIDATKLSRFIEINSLHMVTEYNPVTVIGLFNSVIQIHLLLIMNKASPEYEENMHRYQKAAKLFQGKILFILVDSGMKENGKVISFFKLKESQLPALAIYQTLDDEWDTLPTAEVSVEHVQNFCDGFLSGKLLKENRESEGKTPKVEL
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Predicted Molecular Mass
28.8 kDa
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Molecular Weight
Approximately 32-38 kDa, based on SDS-PAGE under reducing conditions.
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Glycosylation
Yes
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US;may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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