Esterase D/FGH Protein, Human (His)
Based on 1 Customer Validation
Esterase D/FGH Protein, Human (His) is the recombinant human-derived Esterase D/FGH protein, expressed by E. coli, with C-6*His labeled tag.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Esterase D/FGH Protein, Human (His) is the recombinant human-derived Esterase D/FGH protein, expressed by E. coli, with C-6*His labeled tag.
Background
S-formylglutathione hydrolase plays a key role in the detoxification of formaldehyde in prokaryotes and eukaryotes, catalyzing the hydrolysis of S-formylglutathione to formic acid and glutathione. ESD encodes a serine hydrolase belonging to the esterase D family. The encoded enzyme is active against many substrates, including O-acetylated sialic acid, and may be involved in the recycling of sialic acid. It is also used as a genetic marker for retinoblastoma and Wilson's disease. ESD can promote its interaction with metallothionein 2A (MT2A), reduce MT2A protein levels, resulting in upregulation of free zinc ion concentration, and inhibit the migration of A549 lung cancer cells in vitro[1][2][3].
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag C-6*His
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Accession
AAH01169.1 (M1-A282)
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Molecular Construction
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N-term
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FGH (M1-A282)
Accession # AAH01169.1 -
6*His
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C-term
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Protein Length
Full Length
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Synonyms
ESD; Methylumbelliferyl-Acetate Deacetylase; Esterase D; FGH; S-Formylglutathione Hydrolase; Testicular Tissue Protein Li 66; Esterase D/Formylglutathione Hydrolase; Esterase 10
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AA Sequence
MALKQISSNKCFGGLQKVFEHDSVELNCKMKFAVYLPPKAETGKCPALYWLSGLTCTEQNFISKSGYHQSASEHGLVVIAPDTSPRGCNIKGEDESWDFGTGAGFYVDATEDPWKTNYRMYSYVTEELPQLINANFPVDPQRMSIFGHSMGGHGALICALKNPGKYKSVSAFAPICNPVLCPWGKKAFSGYLGTDQSKWKAYDATHLVKSYPGSQLDILIDQGKDDQFLLDGQLLPDNFIAACTEKKIPVVFRLQEDYDHSYYFIATFITDHIRHHAKYLNA
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Predicted Molecular Mass
32.6 kDa
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Molecular Weight
Approximately 31 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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