Esterase D/FGH Protein, Human (His)

Esterase D/FGH Protein, Human (His) is the recombinant human-derived Esterase D/FGH protein, expressed by E. coli, with C-6*His labeled tag.

S-formylglutathione hydrolase plays a key role in the detoxification of formaldehyde in prokaryotes and eukaryotes, catalyzing the hydrolysis of S-formylglutathione to formic acid and glutathione. ESD encodes a serine hydrolase belonging to the esterase D family. The encoded enzyme is active against many substrates, including O-acetylated sialic acid, and may be involved in the recycling of sialic acid. It is also used as a genetic marker for retinoblastoma and Wilson's disease. ESD can promote its interaction with metallothionein 2A (MT2A), reduce MT2A protein levels, resulting in upregulation of free zinc ion concentration, and inhibit the migration of A549 lung cancer cells in vitro^[1][2][3].

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

C-6*His

AAH01169.1 (M1-A282)

2098  [NCBI]

Full Length

MALKQISSNKCFGGLQKVFEHDSVELNCKMKFAVYLPPKAETGKCPALYWLSGLTCTEQNFISKSGYHQSASEHGLVVIAPDTSPRGCNIKGEDESWDFGTGAGFYVDATEDPWKTNYRMYSYVTEELPQLINANFPVDPQRMSIFGHSMGGHGALICALKNPGKYKSVSAFAPICNPVLCPWGKKAFSGYLGTDQSKWKAYDATHLVKSYPGSQLDILIDQGKDDQFLLDGQLLPDNFIAACTEKKIPVVFRLQEDYDHSYYFIATFITDHIRHHAKYLNA

Approximately 33.0 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 10% glycerol, pH 8.0.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.