EZH2 Protein, Human (sf9, His)
Based on 1 publication(s) in Google Scholar
The EZH2 protein is a member of the Polycomb group (PcG). EZH2 Protein, Human (sf9, His) is the recombinant human-derived EZH2, expressed by Sf9 insect cells, with C-10*His labeled tag.
- Species: Human
- Source: Sf9 insect cells
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
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Biological Activity
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Technical Parameters
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Product Properties
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Biological Activity
Description
The EZH2 protein is a member of the Polycomb group (PcG). EZH2 Protein, Human (sf9, His) is the recombinant human-derived EZH2, expressed by Sf9 insect cells, with C-10*His labeled tag.
Background
EZH2, a Polycomb group (PcG) protein, acts as the catalytic subunit of the PRC2/EED-EZH2 complex, contributing to histone methylation and subsequent transcriptional repression. This complex mono-, di-, and trimethylates 'Lys-27' (H3K27) of histone H3, generating H3K27me1, H3K27me2, and H3K27me3, respectively. EZH2 exhibits a preference for substrates with less methylation and is pivotal for forming H3K27me3 in embryonic stem cells, playing a crucial role in maintaining stem cell identity and facilitating proper differentiation. Moreover, the PRC2/EED-EZH2 complex may serve as a platform for recruiting DNA methyltransferases, establishing a connection between distinct epigenetic repression systems. Target genes repressed by this complex include HOXC8, HOXA9, MYT1, CDKN2A, and retinoic acid-responsive genes. EZH2 extends its methylating activity beyond histones to non-histone proteins like the transcription factor GATA4 and nuclear receptor RORA. Additionally, EZH2 regulates the circadian clock through histone methylation at circadian gene promoters, contributing to CRY1/2-mediated repression of CLOCK-BMAL1-driven transcriptional activation of PER1/2. This multifaceted involvement underscores EZH2's integral role in epigenetic regulation and cellular processes.
Publications (1)
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Journal Impact Factor
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Most Recent
Technical Parameters
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Species Human
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Source Sf9 insect cells
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Tag C-10*His
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Accession
Q15910-1 (M1-P746)
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Gene ID2146
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Molecular Construction
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N-term
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EZH2 (M1-P746)
Accession # Q15910-1 -
10*His
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C-term
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Protein Length
Full Length of Isoform-1
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Synonyms
Histone-lysine N-methyltransferase EZH2; EC:2.1.1.356; ENX-1; Enhancer of zeste homolog 2; Lysine N-methyltransferase 6; EZH2
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AA Sequence
MGQTGKKSEKGPVCWRKRVKSEYMRLRQLKRFRRADEVKSMFSSNRQKILERTEILNQEWKQRRIQPVHILTSVSSLRGTRECSVTSDLDFPTQVIPLKTLNAVASVPIMYSWSPLQQNFMVEDETVLHNIPYMGDEVLDQDGTFIEELIKNYDGKVHGDRECGFINDEIFVELVNALGQYNDDDDDDDGDDPEEREEKQKDLEDHRDDKESRPPRKFPSDKIFEAISSMFPDKGTAEELKEKYKELTEQQLPGALPPECTPNIDGPNAKSVQREQSLHSFHTLFCRRCFKYDCFLHPFHATPNTYKRKNTETALDNKPCGPQCYQHLEGAKEFAAALTAERIKTPPKRPGGRRRGRLPNNSSRPSTPTINVLESKDTDSDREAGTETGGENNDKEEEEKKDETSSSSEANSRCQTPIKMKPNIEPPENVEWSGAEASMFRVLIGTYYDNFCAIARLIGTKTCRQVYEFRVKESSIIAPAPAEDVDTPPRKKKRKHRLWAAHCRKIQLKKDGSSNHVYNYQPCDHPRQPCDSSCPCVIAQNFCEKFCQCSSECQNRFPGCRCKAQCNTKQCPCYLAVRECDPDLCLTCGAADHWDSKNVSCKNCSIQRGSKKHLLLAPSDVAGWGIFIKDPVQKNEFISEYCGEIISQDEADRRGKVYDKYMCSFLFNLNNDFVVDATRKGNKIRFANHSVNPNCYAKVMMVNGDHRIGIFAKRAIQTGEELFFDYRYSQADALKYVGIEREMEIP
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Predicted Molecular Mass
86.73 kDa
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Molecular Weight
Approximately 95 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
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Glycosylation
Yes
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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