Ezrin/EZR Protein, Human
Ezrin (EZR) plays a crucial role in connecting major cytoskeletal structures to the plasma membrane and is particularly critical for the development of microvilli and membrane ruffles in epithelial cells. Ezrin cooperates with PLEKHG6 and is essential for normal macropinocytosis. Ezrin/EZR Protein, Human is the recombinant human-derived Ezrin/EZR protein, expressed by E. coli , with tag free.
- Species: Human
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
Ezrin (EZR) plays a crucial role in connecting major cytoskeletal structures to the plasma membrane and is particularly critical for the development of microvilli and membrane ruffles in epithelial cells. Ezrin cooperates with PLEKHG6 and is essential for normal macropinocytosis. Ezrin/EZR Protein, Human is the recombinant human-derived Ezrin/EZR protein, expressed by E. coli , with tag free.
Background
Ezrin (EZR) likely plays a crucial role in establishing connections between major cytoskeletal structures and the plasma membrane. Particularly in epithelial cells, it is essential for the development of microvilli and membrane ruffles on the apical pole. Additionally, in collaboration with PLEKHG6, Ezrin is necessary for the normal process of macropinocytosis. The protein exhibits a head-to-tail association, bringing together its N-terminal and C-terminal halves, resulting in a closed conformation representing the inactive form. In this state, Ezrin is unable to bind to actin or the membrane, suggesting a regulatory mechanism for its functional activation.
MCE Validation Data
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Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
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Species Human
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Source E. coli
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Tag Tag Free
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Accession
P15311 (M1-L586)
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Molecular Construction
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N-term
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EZR (M1-L586)
Accession # P15311 -
C-term
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Protein Length
Full Length
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Synonyms
EZR; Epididymis Secretory Protein Li 105; Prev. VIL2; HEL-S-105; Villin 2 (Ezrin); Villin-2; Cytovillin 2; CVIL; P81; CVL; Ezrin; Cytovillin
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AA Sequence
MPKPINVRVTTMDAELEFAIQPNTTGKQLFDQVVKTIGLREVWYFGLHYVDNKGFPTWLKLDKKVSAQEVRKENPLQFKFRAKFYPEDVAEELIQDITQKLFFLQVKEGILSDEIYCPPETAVLLGSYAVQAKFGDYNKEVHKSGYLSSERLIPQRVMDQHKLTRDQWEDRIQVWHAEHRGMLKDNAMLEYLKIAQDLEMYGINYFEIKNKKGTDLWLGVDALGLNIYEKDDKLTPKIGFPWSEIRNISFNDKKFVIKPIDKKAPDFVFYAPRLRINKRILQLCMGNHELYMRRRKPDTIEVQQMKAQAREEKHQKQLERQQLETEKKRRETVEREKEQMMREKEELMLRLQDYEEKTKKAERELSEQIQRALQLEEERKRAQEEAERLEADRMAALRAKEELERQAVDQIKSQEQLAAELAEYTAKIALLEEARRRKEDEVEEWQHRAKEAQDDLVKTKEELHLVMTAPPPPPPPVYEPVSYHVQESLQDEGAEPTGYSAELSSEGIRDDRNEEKRITEAEKNERVQRQLLTLSSELSQARDENKRTHNDIIHNENMRQGRDKYKTLRQIRQGNTKQRIDEFEAL
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Predicted Molecular Mass
69.4 kDa
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Molecular Weight
Approximately 80 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (238 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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