FBP1 Protein, Human (HEK293, His)
Based on 2 publication(s) in Google Scholar
The FBP1 protein plays a crucial role in cellular processes by catalyzing the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate, acting as a rate-limiting enzyme in gluconeogenesis. In addition to playing a key role in glucose metabolism, FBP1 also plays an important role in regulating glucose sensing and insulin secretion in pancreatic β cells. FBP1 Protein, Human (HEK293, His) is the recombinant human-derived FBP1 protein, expressed by HEK293 , with C-6*His labeled tag.
- Species: Human
- Source: HEK293
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
The FBP1 protein plays a crucial role in cellular processes by catalyzing the hydrolysis of fructose-1,6-bisphosphate to fructose-6-phosphate, acting as a rate-limiting enzyme in gluconeogenesis. In addition to playing a key role in glucose metabolism, FBP1 also plays an important role in regulating glucose sensing and insulin secretion in pancreatic β cells. FBP1 Protein, Human (HEK293, His) is the recombinant human-derived FBP1 protein, expressed by HEK293 , with C-6*His labeled tag.
Background
The FBP1 protein is a key enzyme that catalyzes the hydrolysis of fructose 1,6-bisphosphate to fructose 6-phosphate, playing a crucial role as a rate-limiting enzyme in gluconeogenesis. Its enzymatic activity is facilitated by divalent cations. FBP1 is integral to the regulation of glucose homeostasis and insulin secretion in pancreatic beta-cells, influencing glucose sensing. Additionally, FBP1 is implicated in the modulation of glycerol gluconeogenesis in the liver. Notably, this protein serves as a significant regulator of appetite and adiposity, as increased expression in the liver following nutrient excess leads to elevated levels of circulating satiety hormones and a reduction in appetite-stimulating neuropeptides. This suggests that FBP1 acts as a feedback mechanism to limit weight gain in response to nutritional abundance.
Verified Bioactivity
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Publications (2)
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Journal Impact Factor
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Most Recent
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Nat Commun
PTPRK regulates glycolysis and de novo lipogenesis to promote hepatocyte metabolic reprogramming in obesity. [Abstract]2024 Nov 4;15(1):9522. PMID: 39496584 -
World J Gastroenterol
FBP1 as a key regulator of focal adhesion kinase-mediated hepatic stellate cell activation: Multi-omics and experimental validation. [Abstract]2025 Jul 28;31(28):107361. PMID: 40741470
Technical Parameters
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Species Human
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Source HEK293
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Tag C-6*His
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Accession
P09467 (A2-Q338)
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Molecular Construction
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N-term
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FBP1 (A2-Q338)
Accession # P09467 -
6*His
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C-term
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Protein Length
Full Length of Mature Protein
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Synonyms
FBP1; Liver FBPase; Prev. FBP; Growth-Inhibiting Protein 17; Fructose-1,6-Bisphosphatase 1; Fructose-Bisphosphatase; D-Fructose-1,6-Bisphosphate 1-Phosphohydrolase 1; Fructose-Bisphosphatase 1; FBPase 1
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AA Sequence
ADQAPFDTDVNTLTRFVMEEGRKARGTGELTQLLNSLCTAVKAISSAVRKAGIAHLYGIAGSTNVTGDQVKKLDVLSNDLVMNMLKSSFATCVLVSEEDKHAIIVEPEKRGKYVVCFDPLDGSSNIDCLVSVGTIFGIYRKKSTDEPSEKDALQPGRNLVAAGYALYGSATMLVLAMDCGVNCFMLDPAIGEFILVDKDVKIKKKGKIYSLNEGYARDFDPAVTEYIQRKKFPPDNSAPYGARYVGSMVADVHRTLVYGGIFLYPANKKSPNGKLRLLYECNPMAYVMEKAGGMATTGKEAVLDVIPTDIHQRAPVILGSPDDVLEFLKVYEKHSAQ
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Predicted Molecular Mass
37.5 kDa
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Molecular Weight
Approximately 35-38 kDa, based on SDS-PAGE under reducing conditions.
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
1.Supplied as a 0.22 μm filtered solution of 20 mM Tris-HCl, 200 mM NaCl, 1 mM DTT, 1 mM EDTA, 10% glycerol, pH 8.0.
2.Supplied as a 0.22 μm filtered solution in 20 mM PB, 200 mM NaCl, 1 mM DTT, 1 mM EDTA, 10% glycerol, pH 8.0.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
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Data Sheet (237 KB)
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SDS (252 KB)
- English - EN (252 KB)
- Français - FR (252 KB)
- Deutsch - DE (252 KB)
- Norwegian - NO (252 KB)
- Español - ES (252 KB)
- Swedish - SV (252 KB)
- Italian - IT (252 KB)
- Korean - KR (252 KB)
- Portuguese - PT (252 KB)
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Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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