FSTL5 Protein, Human (HEK293, His)

FSTL5 is an extracellular matrix-secreted protein. FSTL5 promotes cell proliferation and survival by influencing the Wnt/β-catenin signaling pathway in liver cancer and inhibits the progression of liver cancer by inducing Caspase-dependent apoptosis and regulating Bcl-2 family proteins. FSTL5 Protein, Human (HEK293, His) is the recombinant human-derived FSTL5 protein, expressed by HEK293 , with C-His labeled tag.

FSTL5 is an extracellular matrix-secreted protein with a molecular structure similar to follicular listatin (FST). Follistatin-like (FSTL) family proteins are involved in cell migration, proliferation, differentiation and organ development. FST can bind to transforming growth factor β (TGF-β) family activating proteins to promote hepatocyte apoptosis and inhibit hepatocyte proliferation. FST can directly bind to TGF-β3 and inhibit EMT in mammary epithelial cells. Down-regulation of FSTL5 promotes cell proliferation and survival by influencing Wnt/β-catenin signaling pathway in liver cancer and inhibits liver cancer progression by inducing Caspase-dependent apoptosis and regulating Bcl-2 family proteins^[1][2][3].

Measured by its ability to inhibit HepG2 human hepatocellular carcinoma cell survival. The ED₅₀ for this effect is 3.201 μg/mL, corresponding to a specific activity is 312.402 units/mg.

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  Measured by its ability to inhibit HepG2 human hepatocellular carcinoma cell survival. The ED₅₀ for this effect is 3.201 μg/mL, corresponding to a specific activity is 312.402 units/mg.

Human

HEK293

C-10*His

Q8N475-1 (R21-A847)

56884  [NCBI]

Full Length of Isoform-1 Mature Protein

RPTKEGGYGLKSYQPLMRLRHKQEKNQESSRVKGFMIQDGPFGSCENKYCGLGRHCVTSRETGQAECACMDLCKRHYKPVCGSDGEFYENHCEVHRAACLKKQKITIVHNEDCFFKGDKCKTTEYSKMKNMLLDLQNQKYIMQENENPNGDDISRKKLLVDQMFKYFDADSNGLVDINELTQVIKQEELGKDLFDCTLYVLLKYDDFNADKHLALEEFYRAFQVIQLSLPEDQKLSITAATVGQSAVLSCAIQGTLRPPIIWKRNNIILNNLDLEDINDFGDDGSLYITKVTTTHVGNYTCYADGYEQVYQTHIFQVNVPPVIRVYPESQAREPGVTASLRCHAEGIPKPQLGWLKNGIDITPKLSKQLTLQANGSEVHISNVRYEDTGAYTCIAKNEAGVDEDISSLFVEDSARKTLANILWREEGLGIGNMFYVFYEDGIKVIQPIECEFQRHIKPSEKLLGFQDEVCPKAEGDEVQRCVWASAVNVKDKFIYVAQPTLDRVLIVDVQSQKVVQAVSTDPVPVKLHYDKSHDQVWVLSWGTLEKTSPTLQVITLASGNVPHHTIHTQPVGKQFDRVDDFFIPTTTLIITHMRFGFILHKDEAALQKIDLETMSYIKTINLKDYKCVPQSLAYTHLGGYYFIGCKPDSTGAVSPQVMVDGVTDSVIGFNSDVTGTPYVSPDGHYLVSINDVKGLVRVQYITIRGEIQEAFDIYTNLHISDLAFQPSFTEAHQYNIYGSSSTQTDVLFVELSSGKVKMIKSLKEPLKAEEWPWNRKNRQIQDSGLFGQYLMTPSKDSLFILDGRLNKLNCEITEVEKGNTVIWVGDA

Approximately 95 kDa due to the glycosylation

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  ≥ 95%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. /biology-dictionary/hepg2-cell.html  [Content Brief]

  [2]. Zhang D, et al. Down-regulated FSTL5 promotes cell proliferation and survival by affecting Wnt/β-catenin signaling in hepatocellular carcinoma. Int J Clin Exp Pathol. 2015 Mar 1;8(3):3386-94.  [Content Brief]

  [3]. Li C, et al. Follistatin-like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase-dependent apoptosis and regulating Bcl-2 family proteins. J Cell Mol Med. 2018 Dec;22(12):6190-6201.  [Content Brief]

  [4]. Zhang DY, et al. Follistatin Like 5 (FSTL5) inhibits epithelial to mesenchymal transition in hepatocellular carcinoma. Chin Med J (Engl). 2020 Aug 5;133(15):1798-1804.  [Content Brief]