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FSTL5 Protein, Human (HEK293, His)

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Based on 1 Customer Validation

FSTL5 is an extracellular matrix-secreted protein. FSTL5 promotes cell proliferation and survival by influencing the Wnt/β-catenin signaling pathway in liver cancer and inhibits the progression of liver cancer by inducing Caspase-dependent apoptosis and regulating Bcl-2 family proteins. FSTL5 Protein, Human (HEK293, His) is the recombinant human-derived FSTL5 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for FSTL5 Protein, Human (HEK293, His)
  • Experimental Validation Results for FSTL5 Protein, Human (HEK293, His)
  • FSTL5 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • References
  • Help & FAQs
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Biological Activity

Description

FSTL5 is an extracellular matrix-secreted protein. FSTL5 promotes cell proliferation and survival by influencing the Wnt/β-catenin signaling pathway in liver cancer and inhibits the progression of liver cancer by inducing Caspase-dependent apoptosis and regulating Bcl-2 family proteins. FSTL5 Protein, Human (HEK293, His) is the recombinant human-derived FSTL5 protein, expressed by HEK293 , with C-His labeled tag.

Background

FSTL5 is an extracellular matrix-secreted protein with a molecular structure similar to follicular listatin (FST). Follistatin-like (FSTL) family proteins are involved in cell migration, proliferation, differentiation and organ development. FST can bind to transforming growth factor β (TGF-β) family activating proteins to promote hepatocyte apoptosis and inhibit hepatocyte proliferation. FST can directly bind to TGF-β3 and inhibit EMT in mammary epithelial cells. Down-regulation of FSTL5 promotes cell proliferation and survival by influencing Wnt/β-catenin signaling pathway in liver cancer and inhibits liver cancer progression by inducing Caspase-dependent apoptosis and regulating Bcl-2 family proteins[1][2][3].

Verified Bioactivity

Measured by its ability to inhibit HepG2 human hepatocellular carcinoma cell survival. The ED50 for this effect is 3.201 μg/mL, corresponding to a specific activity is 312.402 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-10*His
  • Accession

    Q8N475-1 (R21-A847)

  • Gene ID
    56884  [NCBI]
  • Molecular Construction
    • N-term
    • FSTL5 (R21-A847)
      Accession # Q8N475-1
    • His
    • C-term
  • Protein Length

    Full Length of Isoform-1

  • Synonyms

    FSTL5; Follistatin-Like Protein 5; Follistatin Like 5; DKFZp566D234; KIAA1263; Follistatin-Like 5; Follistatin-Related Protein 5

  • AA Sequence

    RPTKEGGYGLKSYQPLMRLRHKQEKNQESSRVKGFMIQDGPFGSCENKYCGLGRHCVTSRETGQAECACMDLCKRHYKPVCGSDGEFYENHCEVHRAACLKKQKITIVHNEDCFFKGDKCKTTEYSKMKNMLLDLQNQKYIMQENENPNGDDISRKKLLVDQMFKYFDADSNGLVDINELTQVIKQEELGKDLFDCTLYVLLKYDDFNADKHLALEEFYRAFQVIQLSLPEDQKLSITAATVGQSAVLSCAIQGTLRPPIIWKRNNIILNNLDLEDINDFGDDGSLYITKVTTTHVGNYTCYADGYEQVYQTHIFQVNVPPVIRVYPESQAREPGVTASLRCHAEGIPKPQLGWLKNGIDITPKLSKQLTLQANGSEVHISNVRYEDTGAYTCIAKNEAGVDEDISSLFVEDSARKTLANILWREEGLGIGNMFYVFYEDGIKVIQPIECEFQRHIKPSEKLLGFQDEVCPKAEGDEVQRCVWASAVNVKDKFIYVAQPTLDRVLIVDVQSQKVVQAVSTDPVPVKLHYDKSHDQVWVLSWGTLEKTSPTLQVITLASGNVPHHTIHTQPVGKQFDRVDDFFIPTTTLIITHMRFGFILHKDEAALQKIDLETMSYIKTINLKDYKCVPQSLAYTHLGGYYFIGCKPDSTGAVSPQVMVDGVTDSVIGFNSDVTGTPYVSPDGHYLVSINDVKGLVRVQYITIRGEIQEAFDIYTNLHISDLAFQPSFTEAHQYNIYGSSSTQTDVLFVELSSGKVKMIKSLKEPLKAEEWPWNRKNRQIQDSGLFGQYLMTPSKDSLFILDGRLNKLNCEITEVEKGNTVIWVGDA

  • Molecular Weight

    Approximately 95 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

References

[1]. Zhang D, et al. Down-regulated FSTL5 promotes cell proliferation and survival by affecting Wnt/β-catenin signaling in hepatocellular carcinoma. Int J Clin Exp Pathol. 2015 Mar 1;8(3):3386-94. [Content Brief]

[2]. Li C, et al. Follistatin-like protein 5 inhibits hepatocellular carcinoma progression by inducing caspase-dependent apoptosis and regulating Bcl-2 family proteins. J Cell Mol Med. 2018 Dec;22(12):6190-6201. [Content Brief]

[3]. Zhang DY, et al. Follistatin Like 5 (FSTL5) inhibits epithelial to mesenchymal transition in hepatocellular carcinoma. Chin Med J (Engl). 2020 Aug 5;133(15):1798-1804. [Content Brief]

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit HepG2 human hepatocellular carcinoma cell survival. The ED50 for this effect is 3.201 μg/mL, corresponding to a specific activity is 312.402 units/mg.

  • FSTL5 Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for FSTL5 Protein, Human (HEK293, His)
  • Experimental Validation Results for FSTL5 Protein, Human (HEK293, His)
  • FSTL5 Protein, Human (HEK293, His) Product Image