FUBP1 Protein, Human (HEK293, C-His)
Based on 1 Customer Validation
FUBP1 Protein, a pivotal regulator of MYC expression, binds to the far-upstream element (FUSE) upstream of the MYC promoter, dynamically acting as both an activator and repressor of gene expression. It forms a complex with PUF60, associated with FUSE DNA, implying involvement in intricate molecular processes. Interaction with PUF60 and JTV1 suggests potential cooperative actions in gene expression modulation. FUBP1 Protein, Human (HEK293, C-His) is the recombinant human-derived FUBP1 protein, expressed by HEK293 , with C-10*His labeled tag.
- Species: Human
- Source: HEK293
-
Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
FUBP1 Protein, a pivotal regulator of MYC expression, binds to the far-upstream element (FUSE) upstream of the MYC promoter, dynamically acting as both an activator and repressor of gene expression. It forms a complex with PUF60, associated with FUSE DNA, implying involvement in intricate molecular processes. Interaction with PUF60 and JTV1 suggests potential cooperative actions in gene expression modulation. FUBP1 Protein, Human (HEK293, C-His) is the recombinant human-derived FUBP1 protein, expressed by HEK293 , with C-10*His labeled tag.
Background
FUBP1 protein functions as a key regulator of MYC expression, exerting its control by binding to the single-stranded far-upstream element (FUSE) located upstream of the MYC promoter. Its role in transcriptional regulation is dynamic, as it can act both as an activator and repressor of gene expression. FUBP1 forms a complex with PUF60, and this complex is associated with the far upstream element (FUSE) DNA segment, suggesting its involvement in intricate molecular processes. Moreover, FUBP1 interacts with PUF60 and JTV1, indicating potential cooperative actions in the modulation of gene expression.
MCE Validation Data
-
Purity - SDS-PAGE
Purity - SDS-PAGE
Technical Parameters
-
Species Human
-
Source HEK293
-
Tag C-10*His
-
Accession
Q96AE4-1 (M1-Q644)
-
Gene ID8880
-
Molecular Construction
-
N-term
-
FUBP1 (M1-Q644)
Accession # Q96AE4 -
10*His
-
C-term
-
-
Protein Length
Full Length of Isoform-1
-
Synonyms
FUBP1; FUSE Binding Protein 1; Prev. FUBP; HDH V; FBP; Epididymis Secretory Sperm Binding Protein; Far Upstream Element-Binding Protein 1; FUSE-Binding Protein 1; Far Upstream Element (FUSE) Binding Protein 1; FUBP1 Protein; Far Upstream Element Binding P
-
AA Sequence
MADYSTVPPPSSGSAGGGGGGGGGGGVNDAFKDALQRARQIAAKIGGDAGTSLNSNDYGYGGQKRPLEDGDQPDAKKVAPQNDSFGTQLPPMHQQQSRSVMTEEYKVPDGMVGFIIGRGGEQISRIQQESGCKIQIAPDSGGLPERSCMLTGTPESVQSAKRLLDQIVEKGRPAPGFHHGDGPGNAVQEIMIPASKAGLVIGKGGETIKQLQERAGVKMVMIQDGPQNTGADKPLRITGDPYKVQQAKEMVLELIRDQGGFREVRNEYGSRIGGNEGIDVPIPRFAVGIVIGRNGEMIKKIQNDAGVRIQFKPDDGTTPERIAQITGPPDRCQHAAEIITDLLRSVQAGNPGGPGPGGRGRGRGQGNWNMGPPGGLQEFNFIVPTGKTGLIIGKGGETIKSISQQSGARIELQRNPPPNADPNMKLFTIRGTPQQIDYARQLIEEKIGGPVNPLGPPVPHGPHGVPGPHGPPGPPGPGTPMGPYNPAPYNPGPPGPAPHGPPAPYAPQGWGNAYPHWQQQAPPDPAKAGTDPNSAAWAAYYAHYYQQQAQPPPAAPAGAPTTTQTNGQGDQQNPAPAGQVDYTKAWEEYYKKMGQAVPAPTGAPPGGQPDYSAAWAEYYRQQAAYYAQTSPQGMPQHPPAPQGQ
-
Predicted Molecular Mass
67.6 kDa
-
Molecular Weight
Approximately 95 kDa, based on SDS-PAGE under reducing conditions.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder
1.Lyophilized from a 0.22 μm filtered solution of 25 mM Tris-HCl, 100 mM glycine, pH 7.3, 10% glycerol.
2.Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.
Please refer to the lot-specific COA for specific buffer information.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
-
Data Sheet (239 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
