fum Protein, Corynebacterium glutamicum (His)
Fum proteins crucially catalyze the interconversion of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, maintaining metabolite flow in cellular energy metabolism. This critical step, if precisely regulated, helps generate energy and generates intermediates critical to cellular processes. fum Protein, Corynebacterium glutamicum (His) is the recombinant fum protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
Fum proteins crucially catalyze the interconversion of fumarate to L-malate in the tricarboxylic acid (TCA) cycle, maintaining metabolite flow in cellular energy metabolism. This critical step, if precisely regulated, helps generate energy and generates intermediates critical to cellular processes. fum Protein, Corynebacterium glutamicum (His) is the recombinant fum protein, expressed by E. coli , with N-6*His labeled tag.
Background
The fum Protein plays a crucial role in the tricarboxylic acid (TCA) cycle, where it catalyzes the stereospecific interconversion of fumarate to L-malate. This enzymatic activity is pivotal for maintaining the flow of metabolites within the TCA cycle, a central pathway in cellular energy metabolism. The conversion of fumarate to L-malate by fum is a key step that contributes to the production of energy and the generation of intermediates essential for various cellular processes. The precise regulation of this enzymatic reaction highlights the significance of fum in orchestrating metabolic flux and energy production within the cell.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His
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Accession
BAD30011.1 (M1-F469)
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Gene ID/
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Molecular Construction
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N-term
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6*His
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fum (M1-F469)
Accession # BAD30011.1 -
C-term
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Protein Length
Full Length
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Synonyms
Fumarate hydratase class II; EC:4.2.1.2; Fumarase C; Aerobic fumarase; Iron-independent fumarase; fum; aspA; fumC; AUP69_04670
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AA Sequence
MTEQEFRIEHDTMGEVKVPAKALWQAQTQRAVENFPISGRGLESAQIRAMGLLKAACAQVNKDSGALDAEKADAIIAAGKEIASGKHDAEFPIDVFQTGSGTSSNMNTNEVIASIAKANGVEVHPNDHVNMGQSSNDTFPTATHVAATEAAVNDLIPGLKVLHESLAKKANEWSEVVKSGRTHLMDAVPVTLGQEFGGYARQIQLGIERVEATLPRLGELAIGGTAAGTGINTSADFGGKVVAELINLTDVKELKEAENHFEAQAARDALVEFSGAMRVIAVSLYKIANDIRLMGSGPLTGLGEIRLPDLQPGSSIMPGKVNPVLCETATQVSAQVIGNDAAVAFSGTQGQFELNVFIPVMARNVLESARLLANTSRVFATRLVDGIEPNEAHMKELAESSPSIVTPLNSAIGYEAAAKVAKTALAEGKTIRQTVIDLGLVDGEKLTEEELDKRLDVLAMAHTERENKF
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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