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G-CSFR/CD114 Protein, Human (HEK293)

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Based on 1 Customer Validation

The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with tag free.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for G-CSFR/CD114 Protein, Human (HEK293)
  • Experimental Validation Results for G-CSFR/CD114 Protein, Human (HEK293)
  • G-CSFR/CD114 Protein, Human (HEK293) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

The G-CSFR/CD114 protein, as a receptor for granulocyte colony-stimulating factor (CSF3), is essential for granulocyte maturation and plays a key role in the proliferation, differentiation, and survival of neutrophil lineage cells. Furthermore, it may be involved in cell surface adhesion or recognition events. G-CSFR/CD114 Protein, Human (HEK293) is the recombinant human-derived G-CSFR/CD114 protein, expressed by HEK293 , with tag free.

Background

G-CSFR/CD114, the receptor for granulocyte colony-stimulating factor (CSF3), holds a pivotal role in orchestrating granulocytic maturation by governing the proliferation, differentiation, and survival of cells along the neutrophilic lineage. This homodimeric receptor engages with two CSF3 molecules, forming a crucial interaction that regulates essential cellular processes. Beyond its involvement in cellular maturation, G-CSFR/CD114 may contribute to adhesion or recognition events at the cell surface. Furthermore, its interaction with CEACAM1 serves as a regulatory axis, down-regulating the CSF3R-STAT3 pathway by recruiting PTPN6, which subsequently dephosphorylates CSF3R and modulates cellular signaling dynamics.

Verified Bioactivity

Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS-60 cells. The ED50 for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - Cell-Based Assay

    Bioactivity - Cell-Based Assay

    Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS‑60 cells. The ED50 for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag Tag Free
  • Accession

    Q99062-1/NP_000751.1 (E25-P621)

  • Gene ID
    1441  [NCBI]
  • Molecular Construction
    • N-term
    • CSF3R (E25-P621)
      Accession # Q99062-1/NP_000751.1
    • C-term
  • Protein Length

    Partial Extracellular Domain

  • Synonyms

    CSF3R; GCSFR; Prev. CD114; G-CSF Receptor; Granulocyte Colony-Stimulating Factor Receptor; CD114 Antigen; G-CSF-R; SCN7; Colony Stimulating Factor 3 Receptor; Colony Stimulating Factor 3 Receptor (Granulocyte)

  • AA Sequence

    ECGHISVSAPIVHLGDPITASCIIKQNCSHLDPEPQILWRLGAELQPGGRQQRLSDGTQESIITLPHLNHTQAFLSCCLNWGNSLQILDQVELRAGYPPAIPHNLSCLMNLTTSSLICQWEPGPETHLPTSFTLKSFKSRGNCQTQGDSILDCVPKDGQSHCCIPRKHLLLYQNMGIWVQAENALGTSMSPQLCLDPMDVVKLEPPMLRTMDPSPEAAPPQAGCLQLCWEPWQPGLHINQKCELRHKPQRGEASWALVGPLPLEALQYELCGLLPATAYTLQIRCIRWPLPGHWSDWSPSLELRTTERAPTVRLDTWWRQRQLDPRTVQLFWKPVPLEEDSGRIQGYVVSWRPSGQAGAILPLCNTTELSCTFHLPSEAQEVALVAYNSAGTSRPTPVVFSESRGPALTRLHAMARDPHSLWVGWEPPNPWPQGYVIEWGLGPPSASNSNKTWRMEQNGRATGFLLKENIRPFQLYEIIVTPLYQDTMGPSQHVYAYSQEMAPSHAPELHLKHIGKTWAQLEWVPEPPELGKSPLTHYTIFWTNAQNQSFSAILNASSRGFVLHGLEPASLYHIHLMAASQAGATNSTVLTLMTLTP

  • Molecular Weight

    Approximately 85 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.2 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its ability to inhibit the G-CSF-induced proliferation of M-NFS‑60 cells. The ED50 for this effect is 1.299 μg/mL in the presence of 0.125 ng/mL of recombinant human G-CSF, corresponding to a specific activity is 769.82 units/mg.

  • G-CSFR/CD114 Protein, Human (HEK293) Product Image
    1/16
  • Experimental Validation Results for G-CSFR/CD114 Protein, Human (HEK293)
  • Experimental Validation Results for G-CSFR/CD114 Protein, Human (HEK293)
  • G-CSFR/CD114 Protein, Human (HEK293) Product Image