GARS Protein, Human (sf9, His)
Based on 1 Customer Validation
The GARS protein performs ATP-dependent ligation of glycine to its tRNA, creating a Gly-AMP intermediate. It also produces diadenosine tetraphosphate (Ap4A) by condensing two ATP molecules, which is important for cellular regulation. GARS may have a specific role in maintaining Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag.
- Species: Human
- Source: Sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
The GARS protein performs ATP-dependent ligation of glycine to its tRNA, creating a Gly-AMP intermediate. It also produces diadenosine tetraphosphate (Ap4A) by condensing two ATP molecules, which is important for cellular regulation. GARS may have a specific role in maintaining Ap4A homeostasis. GARS Protein, Human (sf9, His) is the recombinant human-derived GARS protein, expressed by Sf9 insect cells , with N-His labeled tag.
GARS, a pivotal enzyme, facilitates the ATP-dependent ligation of glycine to the 3'-end of its corresponding tRNA through the formation of an aminoacyl-adenylate intermediate (Gly-AMP). This process is crucial for protein synthesis and maintaining the fidelity of the genetic code. Additionally, GARS exhibits a unique capability to generate diadenosine tetraphosphate (Ap4A), a universal signaling molecule involved in diverse cell regulation pathways, by directly condensing two ATP molecules. This dual enzymatic activity underscores the multifaceted role of GARS in both fundamental cellular processes, such as protein synthesis, and the modulation of signaling cascades through Ap4A, suggesting its potential significance in Ap4A homeostasis.
The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
Technical Parameters
-
Species Human
-
Source Sf9 insect cells
-
Tag N-His
-
Accession
P41250-1/NP_002038.2 (M1-E739)
-
Molecular Construction
-
N-term
-
His
-
GARS (M1-E739)
Accession # P41250-1/NP_002038.2 -
C-term
-
-
Protein Length
Full Length of Isoform-1
-
Synonyms
GARS1; Charcot-Marie-Tooth Neuropathy 2D; Prev. GARS; Charcot-Marie-Tooth Neuropathy, Neuronal Type, D; Prev. CMT2D; Glycine TRNA Ligase; GlyRS; AP-4-A Synthetase; Diadenosine Tetraphosphate Synthetase; HMN5A; Glycyl-TRNA Synthetase; HMND5; Glycine--TRNA
-
AA Sequence
MPSPRPVLLRGARAALLLLLPPRLLARPSLLLRRSLSAASCPPISLPAAASRSSMDGAGAEEVLAPLRLAVRQQGDLVRKLKEDKAPQVDVDKAVAELKARKRVLEAKELALQPKDDIVDRAKMEDTLKRRFFYDQAFAIYGGVSGLYDFGPVGCALKNNIIQTWRQHFIQEEQILEIDCTMLTPEPVLKTSGHVDKFADFMVKDVKNGECFRADHLLKAHLQKLMSDKKCSVEKKSEMESVLAQLDNYGQQELADLFVNYNVKSPITGNDLSPPVSFNLMFKTFIGPGGNMPGYLRPETAQGIFLNFKRLLEFNQGKLPFAAAQIGNSFRNEISPRSGLIRVREFTMAEIEHFVDPSEKDHPKFQNVADLHLYLYSAKAQVSGQSARKMRLGDAVEQGVINNTVLGYFIGRIYLYLTKVGISPDKLRFRQHMENEMAHYACDCWDAESKTSYGWIEIVGCADRSCYDLSCHARATKVPLVAEKPLKEPKTVNVVQFEPSKGAIGKAYKKDAKLVMEYLAICDECYITEMEMLLNEKGEFTIETEGKTFQLTKDMINVKRFQKTLYVEEVVPNVIEPSFGLGRIMYTVFEHTFHVREGDEQRTFFSFPAVVAPFKCSVLPLSQNQEFMPFVKELSEALTRHGVSHKVDDSSGSIGRRYARTDEIGVAFGVTIDFDTVNKTPHTATLRDRDSMRQIRAEISELPSIVQDLANGNITWADVEARYPLFEGQETGKKETIEE
-
Predicted Molecular Mass
85.5 kDa
-
Purity
≥ 85%, as determined by reducing SDS-PAGE.
Product Properties
Solution
Supplied as a 0.22 μm filtered solution of 20 mM Tris, pH 7.5, 150 mM Nacl, 20% glycerol.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
-
Data Sheet (239 KB)
-
SDS (251 KB)
- English - EN (251 KB)
- Français - FR (251 KB)
- Deutsch - DE (251 KB)
- Norwegian - NO (251 KB)
- Español - ES (251 KB)
- Swedish - SV (251 KB)
- Italian - IT (251 KB)
- Korean - KR (251 KB)
- Portuguese - PT (251 KB)
-
Handling Instructions (2659 KB)
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
