glycoprotein/G Protein, HRSV (His)

Glycoproteins/G proteins play a key role in infection by attaching viral particles to the host cell membrane through heparan sulfate interactions, thereby initiating infection. It also interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, contributing to immune regulation and promoting infection. glycoprotein/G Protein, HRSV (His) is the recombinant virus-derived glycoprotein/G protein, expressed by E. coli, with N-His labeled tag.

The glycoprotein/G protein plays a pivotal role in infection by attaching the virion to the host cell membrane through interaction with heparan sulfate, initiating the infection. Additionally, it interacts with host CX3CR1, the receptor for the CX3C chemokine fractalkine, contributing to immune modulation and facilitating infection. Distinguished from other paramyxovirus attachment proteins, the glycoprotein lacks both neuraminidase and hemagglutinating activities. Its unique features enable the virus to evade antibody-dependent restriction of replication by functioning as an antigen decoy and modulating the activity of leukocytes carrying Fc-gamma receptors.

Virus

E. coli

N-His

O36633-1 (H67-A299)

1489824

Full Length of Mature secreted glycoprotein G

HKVTLTTVTVQTIKNHTEKNITTYLTQVPPERVSSSKQPTTTSPIHTNSATTSPNTKSETHHTTAQTKGRTTTSTQTNKPSTKPRLKNPPKKPKDDYHFEVFNFVPCSICGNNQLCKSICKTIPSNKPKKKPTIKPTNKPTTKTTNKRDPKTPAKTTKKETTTNPTKKPTLTTTERDTSTSQSTVLDTTTLEHTIQQQSLHSTTPENTPNSTQTPTASEPSTSNSTQNTQSHA

Approximately 33-35 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized from 0.22 μm filtered solution of PBS, pH7.4 with trehalose as protectant.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.