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HAI-1 Protein, Human (HEK293, His)

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HAI-1 Protein serves as an inhibitor of HGFAC, suppressing serine protease activity of ST14/matriptase in vitro and inhibiting TMPRSS13 through the BPTI/Kunitz inhibitor 1 domain. It interacts with HGFAC and TMPRSS13, with the latter interaction promoting TMPRSS13 phosphorylation and cell membrane localization. HAI-1 Protein, Human (HEK293, His) is the recombinant human-derived HAI-1 protein, expressed by HEK293 , with C-His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • HAI-1 Protein, Human (HEK293, His) Product Image
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To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

HAI-1 Protein serves as an inhibitor of HGFAC, suppressing serine protease activity of ST14/matriptase in vitro and inhibiting TMPRSS13 through the BPTI/Kunitz inhibitor 1 domain. It interacts with HGFAC and TMPRSS13, with the latter interaction promoting TMPRSS13 phosphorylation and cell membrane localization. HAI-1 Protein, Human (HEK293, His) is the recombinant human-derived HAI-1 protein, expressed by HEK293 , with C-His labeled tag.

Background

The HAI-1 Protein functions as an inhibitor of HGFAC and exhibits inhibitory effects on the serine protease activity of ST14/matriptase in vitro. Through its BPTI/Kunitz inhibitor 1 domain, HAI-1 also impedes the serine protease activity of TMPRSS13. Interacting with HGFAC and TMPRSS13, HAI-1 forms dynamic associations that influence cellular processes. The interaction with HGFAC suggests a regulatory role in the activation of this protease, while the interaction with TMPRSS13 promotes the phosphorylation and cell membrane localization of TMPRSS13. These intricate interactions and inhibitory functions highlight the versatile regulatory role of HAI-1 in modulating serine protease activities, underscoring its significance in cellular homeostasis and proteolytic pathways.

Verified Bioactivity

Measured by its ability to inhibit trypsin cleavage of a fluorogenic peptide substrate, Mca-Arg-Pro-Lys-Pro-Val-Glu-NVAL-Trp-Arg-Lys(DNP)-NH2. The IC50 is < 1 nM.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-His
  • Accession

    O43278-2 (G36-V433)

  • Gene ID
    6692  [NCBI]
  • Molecular Construction
    • N-term
    • HAI-1 (G36-V433)
      Accession # O43278-2
    • His
    • C-term
  • Protein Length

    Partial

  • Synonyms

    SPINT1; MANSC2; Serine Peptidase Inhibitor, Kunitz Type 1; HAI; HAI-1; Serine Protease Inhibitor, Kunitz Type 1; Hepatocyte Growth Factor Activator Inhibitor Type 1; HAI1; Kunitz-Type Protease Inhibitor 1

  • AA Sequence

    GPPPAPPGLPAGADCLNSFTAGVPGFVLDTNASVSNGATFLESPTVRRGWDCVRACCTTQNCNLALVELQPDRGEDAIAACFLINCLYEQNFVCKFAPREGFINYLTREVYRSYRQLRTQGFGGSGIPKAWAGIDLKVQPQEPLVLKDVENTDWRLLRGDTDVRVERKDPNQVELWGLKEGTYLFQLTVTSSDHPEDTANVTVTVLSTKQTEDYCLASNKVGRCRGSFPRWYYDPTEQICKSFVYGGCLGNKNNYLREEECILACRGVQGPSMERRHPVCSGTCQPTQFRCSNGCCIDSFLECDDTPNCPDASDEAACEKYTSGFDELQRIHFPSDKGHCVDLPDTGLCKESIPRWYYNPFSEHCARFTYGGCYGNKNNFEEEQQCLESCRGISKKDV

  • Predicted Molecular Mass

    45.5 kDa

  • Molecular Weight

    Approximately 50-70 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

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