HAO1 Protein, Human (Trx-His)

The HAO1 protein is a (S)-2-hydroxyacid oxidase with broad substrate specificity that facilitates glycolate oxidation. This activity produces glyoxylic acid, a key precursor for glycine synthesis. HAO1 Protein, Human (Trx-His) is the recombinant human-derived HAO1 protein, expressed by E. coli , with N-Trx, N-6*His labeled tag. The total length of HAO1 Protein, Human (Trx-His) is 370 a.a., with molecular weight of ~56.0 kDa.

HAO1 Protein exhibits broad substrate specificity as an (S)-2-hydroxy-acid oxidase, with a notable preference for glycolate oxidation. This enzymatic activity generates glyoxylate, a crucial precursor utilized by alanine-glyoxylate aminotransferase in the peroxisomal synthesis of glycine. This pathway plays a vital role in detoxifying glyoxylate, preventing its accumulation, which could otherwise lead to the formation of kidney stones. Additionally, HAO1 can catalyze the oxidation of glyoxylate and long-chain hydroxyacids, such as 2-hydroxyhexadecanoate and 2-hydroxyoctanoate, albeit with lower catalytic efficiency. While active in vitro with the artificial electron acceptor 2,6-dichlorophenolindophenol (DCIP), it is believed that O2 serves as the physiological electron acceptor, leading to the production of H2O2. Notably, HAO1 does not exhibit activity on L-lactate and 2-hydroxybutanoate. This multifaceted enzymatic functionality underscores its importance in diverse metabolic pathways.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Human

E. coli

N-Trx;N-6*His

Q9UJM8 (M1-I370)

54363  [NCBI]

MLPRLICINDYEQHAKSVLPKSIYDYYRSGANDEETLADNIAAFSRWKLYPRMLRNVAETDLSTSVLGQRVSMPICVGATAMQRMAHVDGELATVRACQSLGTGMMLSSWATSSIEEVAEAGPEALRWLQLYIYKDREVTKKLVRQAEKMGYKAIFVTVDTPYLGNRLDDVRNRFKLPPQLRMKNFETSTLSFSPEENFGDDSGLAAYVAKAIDPSISWEDIKWLRRLTSLPIVAKGILRGDDAREAVKHGLNGILVSNHGARQLDGVPATIDVLPEIVEAVEGKVEVFLDGGVRKGTDVLKALALGAKAVFVGRPIVWGLAFQGEKGVQDVLEILKEEFRLAMALSGCQNVKVIDKTLVRKNPLAVSKI

Approximately 56.0 kDa

Greater than 90% as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.2 μm filtered solution of PBS, pH 7.4.

<1 EU/μg, determined by LAL method.

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.