DDC Protein, Human (Sf9, His)
DDC protein plays a central role in neurotransmitter synthesis by catalyzing the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to produce dopamine and L-5-hydroxytryptophan to produce serotonin. DDC Protein, Human (Sf9, His) is the recombinant human-derived DDC protein, expressed by sf9 insect cells, with C-His labeled tag.
- Species: Human
- Source: sf9 insect cells
-
Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
-
Biological Activity
-
Technical Parameters
-
Product Properties
- Documentation
- Help & FAQs
Biological Activity
Description
DDC protein plays a central role in neurotransmitter synthesis by catalyzing the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to produce dopamine and L-5-hydroxytryptophan to produce serotonin. DDC Protein, Human (Sf9, His) is the recombinant human-derived DDC protein, expressed by sf9 insect cells, with C-His labeled tag.
Background
Human aromatic L-amino acid decarboxylase (hDDC) is an enzyme that plays a crucial role in neurotransmitter biosynthesis by catalyzing the decarboxylation of L-3,4-dihydroxyphenylalanine (DOPA) to dopamine and L-5-hydroxytryptophan to serotonin. This enzymatic activity is a pivotal step in the synthesis of two key neurotransmitters, dopamine and serotonin, both of which are essential for the central nervous system's regulation of mood, behavior, and motor functions. The conversion of DOPA to dopamine by hDDC is particularly significant in the dopaminergic pathways, influencing motor control and emotional processes. The parallel conversion of L-5-hydroxytryptophan to serotonin underscores hDDC's role in serotoninergic pathways, crucial for mood and emotional regulation. It has to emphasize hDDC's specific contributions to neurotransmitter synthesis, highlighting its importance in maintaining the delicate balance of signaling molecules critical for neurological functions.
Technical Parameters
-
Species Human
-
Source sf9 insect cells
-
Tag C-His
-
Accession
P20711 (M1-E480)
-
Gene ID1644
-
Molecular Construction
-
N-term
-
hDDC (M1-E480)
Accession # P20711 -
His
-
C-term
-
-
Protein Length
Full Length
-
Synonyms
DDC; DOPA Decarboxylase; Dopa Decarboxylase; Dopa Decarboxylase (Aromatic L-Amino Acid Decarboxylase); AADC; Aromatic L-Amino Acid Decarboxylase; Aromatic-L-Amino-Acid Decarboxylase; Dopa Decarboxylase Isoform 2
-
AA Sequence
MNASEFRRRGKEMVDYMANYMEGIEGRQVYPDVEPGYLRPLIPAAAPQEPDTFEDIINDVEKIIMPGVTHWHSPYFFAYFPTASSYPAMLADMLCGAIGCIGFSWAASPACTELETVMMDWLGKMLELPKAFLNEKAGEGGGVIQGSASEATLVALLAARTKVIHRLQAASPELTQAAIMEKLVAYSSDQAHSSVERAGLIGGVKLKAIPSDGNFAMRASALQEALERDKAAGLIPFFMVATLGTTTCCSFDNLLEVGPICNKEDIWLHVDAAYAGSAFICPEFRHLLNGVEFADSFNFNPHKWLLVNFDCSAMWVKKRTDLTGAFRLDPTYLKHSHQDSGLITDYRHWQIPLGRRFRSLKMWFVFRMYGVKGLQAYIRKHVQLSHEFESLVRQDPRFEICVEVILGLVCFRLKGSNKVNEALLQRINSAKKIHLVPCHLRDKFVLRFAICSRTVESAHVQRAWEHIKELAADVLRAERE
-
Predicted Molecular Mass
55.8 kDa
-
Molecular Weight
Approximately 53-58 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.
-
Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Solution
<1 EU/μg, determined by LAL method.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
Powered by Bioz
