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Heparanase/HPSE Protein, Human (CHO, His)

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Based on 1 Customer Validation

Heparanase (HPSE) functions as an endoglycosidase, playing a crucial role in the cleavage of heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. It is implicated in various cellular processes, including facilitating cell migration associated with metastasis, wound healing, and inflammation. Heparanase also plays a role in cell adhesion to the ECM, independent of its enzymatic activity, and induces AKT1/PKB phosphorylation, thereby increasing cell mobility and invasion. Additionally, Heparanase is involved in the regulation of osteogenesis, promotes angiogenesis through up-regulation of SRC-mediated activation of VEGF, and is implicated in hair follicle inner root sheath differentiation and hair homeostasis. Heparanase/HPSE Protein, Human (CHO, His) is the recombinant human-derived Heparanase/HPSE protein, expressed by CHO , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: CHO
  • Storage:
    Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
  • Experimental Validation Results for Heparanase/HPSE Protein, Human (CHO, His)
  • Experimental Validation Results for Heparanase/HPSE Protein, Human (CHO, His)
  • Heparanase/HPSE Protein, Human (CHO, His) Product Image
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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
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Biological Activity

Description

Heparanase (HPSE) functions as an endoglycosidase, playing a crucial role in the cleavage of heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. It is implicated in various cellular processes, including facilitating cell migration associated with metastasis, wound healing, and inflammation. Heparanase also plays a role in cell adhesion to the ECM, independent of its enzymatic activity, and induces AKT1/PKB phosphorylation, thereby increasing cell mobility and invasion. Additionally, Heparanase is involved in the regulation of osteogenesis, promotes angiogenesis through up-regulation of SRC-mediated activation of VEGF, and is implicated in hair follicle inner root sheath differentiation and hair homeostasis. Heparanase/HPSE Protein, Human (CHO, His) is the recombinant human-derived Heparanase/HPSE protein, expressed by CHO , with N-6*His labeled tag.

Background

Heparanase (HPSE) functions as an endoglycosidase, playing a crucial role in the cleavage of heparan sulfate proteoglycans (HSPGs) into heparan sulfate side chains and core proteoglycans. This enzymatic activity contributes to extracellular matrix (ECM) degradation and remodeling, selectively cleaving linkages within glucuronic acid units and N-sulfo glucosamine units carrying specific sulfation patterns. While predominantly inactive at neutral pH, HPSE becomes active under acidic conditions, such as during tumor invasion and inflammatory processes. This protein is implicated in various cellular processes, including facilitating cell migration associated with metastasis, wound healing, and inflammation. It enhances shedding of syndecans, promotes endothelial invasion and angiogenesis in myelomas, and acts as a procoagulant by increasing the generation of activation factor X in the presence of tissue factor and activation factor VII. Notably, HPSE also plays a role in cell adhesion to the ECM, independent of its enzymatic activity, and induces AKT1/PKB phosphorylation, thereby increasing cell mobility and invasion. Additionally, HPSE is involved in the regulation of osteogenesis, promotes angiogenesis through up-regulation of SRC-mediated activation of VEGF, and is implicated in hair follicle inner root sheath differentiation and hair homeostasis[1][2][3][4][5].

Verified Bioactivity

1.The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.
2.Measured by its binding ability in a functional ELISA. Immobilized HPSE at 2 μg/ml (100μl/well) can bind HPSE antibody. The ED50 for this effect is 0.1-0.5 μg/mL.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

  • Bioactivity - ELISA

    Bioactivity - ELISA

    Measured by its binding ability in a functional ELISA. Immobilized HPSE at 2 μg/mL (100μl/well) can bind HPSE antibody. The ED50 for this effect is 0.2357 μg/mL.

Technical Parameters

  • Species Human
  • Source CHO
  • Tag N-6*His
  • Accession

    AAD41342.1 (Q36-I543)

  • Gene ID
    10855  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • HPSE (Q36-I543)
      Accession # AAD41342.1
    • C-term
  • Protein Length

    Full length of Mature Protein

  • Synonyms

    HPSE; Endo-Glucoronidase; Heparanase; HPA1; HPSE1; HPR1; HSE1; Hpa1; HPA; HEP; Heparanase-1

  • AA Sequence

    QDVVDLDFFTQEPLHLVSPSFLSVTIDANLATDPRFLILLGSPKLRTLARGLSPAYLRFGGTKTDFLIFDPKKESTFEERSYWQSQVNQDICKYGSIPPDVEEKLRLEWPYQEQLLLREHYQKKFKNSTYSRSSVDVLYTFANCSGLDLIFGLNALLRTADLQWNSSNAQLLLDYCSSKGYNISWELGNEPNSFLKKADIFINGSQLGEDFIQLHKLLRKSTFKNAKLYGPDVGQPRRKTAKMLKSFLKAGGEVIDSVTWHHYYLNGRTATREDFLNPDVLDIFISSVQKVFQVVESTRPGKKVWLGETSSAYGGGAPLLSDTFAAGFMWLDKLGLSARMGIEVVMRQVFFGAGNYHLVDENFDPLPDYWLSLLFKKLVGTKVLMASVQGSKRRKLRVYLHCTNTDNPRYKEGDLTLYAINLHNVTKYLRLPYPFSNKQVDKYLLRPLGPHGLLSKSVQLNGLTLKMVDDQTLPPLMEKPLRPGSSLGLPAFSYSFFVIRNAKVAACI

  • Predicted Molecular Mass

    58.4 kDa

  • Molecular Weight

    Approximately 78 kDa, based on SDS-PAGE under reducing conditions, due to the glycosylation.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Lyophilized powder

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 8% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • 1/16

    MedChemExpress Validation
     Measured by its binding ability in a functional ELISA. Immobilized HPSE at 2 μg/mL (100μl/well) can bind HPSE antibody. The ED50 for this effect is 0.2357 μg/mL.

  • Heparanase/HPSE Protein, Human (CHO, His) Product Image
    1/16
  • Experimental Validation Results for Heparanase/HPSE Protein, Human (CHO, His)
  • Experimental Validation Results for Heparanase/HPSE Protein, Human (CHO, His)
  • Heparanase/HPSE Protein, Human (CHO, His) Product Image