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  3. HLA-E*01:03&B2M&Peptide(VMAPRTLVL) Monomer Protein, Human (HEK293, His-Avi)

HLA-E*01:03&B2M&Peptide(VMAPRTLVL) Monomer Protein, Human (HEK293, His-Avi)

Art. -Nr.: HY-P77758
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  • Technical Parameters

  • Properties

  • Beschreibung

Species

Human

Source

HEK293

Tag

C-Avi;C-8*His

Accession

P13747 (G22-T302)&P61769 (I21-M119)&VMAPRTLVL

Gene ID

3133  [NCBI]&567  [NCBI]

Synonyms
HLAE; sHLA-E; HLAE; MHC class I antigen E; MHC HLA-E alpha-1; MHC HLA-E alpha-2.1; MHC; QA1
AA Sequence

P13747(HLA-E*01:03): GSHSLKYFHT
SVSRPGRGEPRFISVGYVDDTQFVRFDNDAASPRMVPRAPWMEQEGSEYWDRETRSARDTAQIFRVNLRTLRGYYNQSEAGSHTLQWMHGCELGPDGRFLRGYEQFAYDGKDYLTLNEDLRSWTAVDTAAQISEQKSNDASEAEHQRAYLEDTCVEWLHKYLEKGKETLLHLEPPKTHVTHHPISDHEATLRCWALGFYPAEITLTWQQDGEGHTQDTELVETRPAGDGTFQKWAAVVVPSGEEQRYTCHVQHEGLPEPVTLRWKPASQPT <br/>P61769
(B2M): IQRTPKIQVY
SRHPAENGKSNFLNCYVSGFHPSDIEVDLLKNGERIEKVEHSDLSFSKDWSFYLLYYTEFTPTEKDEYACRVNHVTLSQPKIVKWDRDM <br/>VMAPRTLVL

Molekulargewicht

52-65 kDa

Glycosylation
Yes
Reinheit

≥ 95%, as determined by Bis-Tris PAGE.

Appearance

Lyophilized powder.

Formulation

Lyophilized from a 0.22 μm filtered solution of PBS, pH 7.4, 5% trehalose.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O.

Storage & Stability

Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Versand

Room temperature in continental US; may vary elsewhere.

Dokumentation

HLA-E*01:03&B2M&Peptide(VMAPRTLVL) Monomer Protein, Human (HEK293, His-Avi) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Activity Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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HLA-E*01:03&B2M&Peptide(VMAPRTLVL) Monomer Protein, Human (HEK293, His-Avi)

 

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Produktname:
HLA-E*01:03&B2M&Peptide(VMAPRTLVL) Monomer Protein, Human (HEK293, His-Avi)
Art. -Nr.:
HY-P77758
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MCE Japan Authorized Agent: