HSPA1L Protein, Human (His, Strep)

HSPA1L Protein, Human (His, Strep) is the recombinant human-derived HSPA1L, expressed by E. coli , with Strep, His labeled tag.

HSP70 is a molecular chaperone involved in a wide range of cellular processes, including protecting the proteome from stress, folding and transporting newly synthesized polypeptides, activating proteolysis of misfolded proteins, and facilitating the formation and dissociation of protein complexes. It plays a central role in the protein quality control system by ensuring proper protein folding, refolding misfolded proteins, and regulating the targeting of proteins for degradation. This function is mediated through cycles of ATP binding, hydrolysis, and ADP release, coordinated by co-chaperones. The affinity of HSP70 for polypeptides is regulated by its nucleotide-bound state: it exhibits low substrate affinity in the ATP-bound form, but undergoes a conformational shift upon ATP hydrolysis to ADP, increasing substrate binding. Repeated cycles of ATP hydrolysis and nucleotide exchange enable substrate binding and release. Additionally, HSP70 positively regulates PRKN translocation to damaged mitochondria.

Human

E. coli

Strep;His

P34931 (A2-D641)

3305

Partial

ATAKGIAIGIDLGTTYSCVGVFQHGKVEIIANDQGNRTTPSYVAFTDTERLIGDAAKNQVAMNPQNTVFDAKRLIGRKFNDPVVQADMKLWPFQVINEGGKPKVLVSYKGENKAFYPEEISSMVLTKLKETAEAFLGHPVTNAVITVPAYFNDSQRQATKDAGVIAGLNVLRIINEPTAAAIAYGLDKGGQGERHVLIFDLGGGTFDVSILTIDDGIFEVKATAGDTHLGGEDFDNRLVSHFVEEFKRKHKKDISQNKRAVRRLRTACERAKRTLSSSTQANLEIDSLYEGIDFYTSITRARFEELCADLFRGTLEPVEKALRDAKMDKAKIHDIVLVGGSTRIPKVQRLLQDYFNGRDLNKSINPDEAVAYGAAVQAAILMGDKSEKVQDLLLLDVAPLSLGLETAGGVMTALIKRNSTIPTKQTQIFTTYSDNQPGVLIQVYEGERAMTKDNNLLGRFDLTGIPPAPRGVPQIEVTFDIDANGILNVTATDKSTGKVNKITITNDKGRLSKEEIERMVLDAEKYKAEDEVQREKIAAKNALESYAFNMKSVVSDEGLKGKISESDKNKILDKCNELLSWLEVNQLAEKDEFDHKRKELEQMCNPIITKLYQGGCTGPACGTGYVPGRPATGPTIEEVD

Approximately 73 kDa, based on SDS-PAGE under reducing conditions.

≥ 90%, as determined by reducing SDS-PAGE.

Solution

Supplied as a 0.22 μm filtered solution of 50 mM HEPES, pH 7.5, 200 mM NaCl, 20% glycerol, 1 mM DTT.

<1 EU/μg, determined by LAL method.

Please use rapid thawing with running water to thaw the protein.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.