hydG Protein, Pyrococcus furiosus (His)
HydG is a key component of a bifunctional enzyme complex that exerts sulfur reducing activity as an NADPH-dependent hydrogen evolution hydrogenase. Its role in hydrogen cycling during fermentative growth highlights its cellular importance. hydG Protein, Pyrococcus furiosus (His) is the recombinant hydG protein, expressed by E. coli , with N-6*His labeled tag.
- Species: Others
- Source: E. coli
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Storage:Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
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Biological Activity
Description
HydG is a key component of a bifunctional enzyme complex that exerts sulfur reducing activity as an NADPH-dependent hydrogen evolution hydrogenase. Its role in hydrogen cycling during fermentative growth highlights its cellular importance. hydG Protein, Pyrococcus furiosus (His) is the recombinant hydG protein, expressed by E. coli , with N-6*His labeled tag.
Background
HydG constitutes a vital component of a bifunctional enzyme complex, operating as an NADPH-dependent hydrogen-evolving hydrogenase with sulfur reducing activity. Its potential involvement in hydrogen cycling during fermentative growth underscores its significance in cellular processes. Intriguingly, HydG's activity is not evident with NAD, and it is the collaborative action of its beta and gamma subunits that forms the sulfur-reducing component, facilitating the cytoplasmic generation of hydrogen sulfide in the presence of elemental sulfur. It's noteworthy that HydG remains inactive when exposed to sodium sulfate, sodium sulfite, sodium thiosulfate, or cysteine, shedding light on its nuanced responsiveness to environmental factors.
Technical Parameters
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Species Others
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Source E. coli
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Tag N-6*His
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Accession
Q8U2E4 (M1-D292)
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Gene ID41712700
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Molecular Construction
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N-term
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6*His
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hydG (M1-D292)
Accession # Q8U2E4 -
C-term
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Protein Length
Full Length
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Synonyms
Sulfhydrogenase 1 subunit gamma; EC:1.12.98.4; Sulfhydrogenase I subunit gamma; Sulfur reductase subunit HydG; hydG; PF0892
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AA Sequence
MMLPKEIMMPNDNPYALHRVKVLKVYSLTETEKLFLFRFEDPELAEKWTFKPGQFVQLTIPGVGEVPISICSSPMRKGFFELCIRKAGRVTTVVHRLKPGDTVLVRGPYGNGFPVDEWEGMDLLLIAAGLGTAPLRSVFLYAMDNRWKYGNITFINTARYGKDLLFYKELEAMKDLAEAENVKIIQSVTRDPNWPGLKGRPQQFIVEANTNPKNTAVAICGPPRMYKSVFEALINYGYRPENIFVTLERRMKCGIGKCGHCNVGTSTSWKYICKDGPVFTYFDIVSTPGLLD
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Purity
≥ 90%, as determined by reducing SDS-PAGE.
Product Properties
Solution.
<1 EU/μg, determined by LAL method.
Please use rapid thawing with running water to thaw the protein.
Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
Shipping with dry ice.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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