1. Recombinant Proteins
  2. Immune Checkpoint Proteins
  3. Inhibitory Checkpoint Molecules
  4. IDO
  5. IDO Protein, Human (His)

IDO Protein, Human (His)

Cat. No.: HY-P70679
COA Handling Instructions

IDO proteins catalyze the first step in tryptophan catabolism via the kynurenine pathway, which is critical for immune tolerance and homeostasis maintenance. IDO Protein, Human (His) is the recombinant human-derived IDO protein, expressed by E. coli , with N-6*His labeled tag. The total length of IDO Protein, Human (His) is 403 a.a., with molecular weight of 40-50 kDa.

For research use only. We do not sell to patients.

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Description

IDO proteins catalyze the first step in tryptophan catabolism via the kynurenine pathway, which is critical for immune tolerance and homeostasis maintenance. IDO Protein, Human (His) is the recombinant human-derived IDO protein, expressed by E. coli , with N-6*His labeled tag. The total length of IDO Protein, Human (His) is 403 a.a., with molecular weight of 40-50 kDa.

Background

The IDO protein plays a central role in cellular processes by catalyzing the initial and rate-limiting step in the catabolism of the essential amino acid tryptophan along the kynurenine pathway, as documented in various studies. Beyond its enzymatic function, IDO is involved in peripheral immune tolerance, contributing to the maintenance of homeostasis by preventing autoimmunity or immunopathology resulting from uncontrolled and overreacting immune responses. Tryptophan scarcity, a consequence of IDO activity, inhibits T lymphocytes division, and the accumulation of tryptophan catabolites induces T-cell apoptosis while promoting the differentiation of regulatory T-cells. Additionally, IDO acts as a suppressor of anti-tumor immunity and limits the growth of intracellular pathogens by depriving them of tryptophan. Furthermore, IDO serves a crucial role in protecting the fetus from maternal immune rejection. These diverse functions underscore the significance of IDO in orchestrating immune responses, maintaining metabolic balance, and contributing to crucial physiological processes with implications for autoimmunity, cancer, and host-pathogen interactions.

Biological Activity

Measured by its ability to oxidize L-tryptophan to N-formyl-kynurenine. The specific activity is 664.3519 pmol/min/µg, as measured under the described conditions.

Species

Human

Source

E. coli

Tag

N-6*His

Accession

P14902 (M1-G403)

Gene ID
Molecular Construction
N-term
6*His
IDO (M1-G403)
Accession # P14902
C-term
Synonyms
Indole 2; 3-dioxygenase; Indoleamine 2; 3-dioxygenase 1; IDO-1; IDO1; IDO; INDO
AA Sequence

AHAMENSWTISKEYHIDEEVGFALPNPQENLPDFYNDWMFIAKHLPDLIESGQLRERVEKLNMLSIDHLTDHKSQRLARLVLGCITMAYVWGKGHGDVRKVLPRNIAVPYCQLSKKLELPPILVYADCVLANWKKKDPNKPLTYENMDVLFSFRDGDCSKGFFLVSLLVEIAAASAIKVIPTVFKAMQMQERDTLLKALLEIASCLEKALQVFHQIHDHVNPKAFFSVLRIYLSGWKGNPQLSDGLVYEGFWEDPKEFAGGSAGQSSVFQCFDVLLGIQQTAGGGHAAQFLQDMRRYMPPAHRNFLCSLESNPSVREFVLSKGDAGLREAYDACVKALVSLRSYHLQIVTKYILIPASQQPKENKTSEDPSKLEAKGTGGTDLMNFLKTVRSTTEKSLLKEG

Molecular Weight

Approximately 40-50 kDa

Purity

Greater than 90% as determined by reducing SDS-PAGE.

Appearance

Solution

Formulation

Supplied as a 0.2 μm filtered solution of 20 mM Sodium Acetate, 150 mM NaCl and 20% Glycerol, pH 4.5 or 50 mM Tris-HCL, 300 mM NaCl, pH 7.4, 20% Glycerol.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

IDO Protein, Human (His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

  • Reconstitution Calculator

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IDO Protein, Human (His)
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