1. Recombinant Proteins
  2. Cytokines and Growth Factors Biotinylated Proteins
  3. Interleukin & Receptors Neurotrophic Factors
  4. IL-1
  5. IL-1 beta
  6. IL-1 beta Protein, Human (Biotinylated, His-Avi)

IL-1 beta Protein, Human (Biotinylated, His-Avi)

Cat. No.: HY-P78143
Handling Instructions

For research use only. We do not sell to patients.

Protein Expression Service

Recombinant Proteins-MedChemExpress
Size Price Stock
20 μg $535 Ask For Quote & Lead Time
50 μg $885 Ask For Quote & Lead Time
100 μg $1500 Ask For Quote & Lead Time

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  • Technical Parameters

  • Properties

  • Documentation

Species

Human

Source

E.coli

Tag

C-Avi;C-His

Accession

P01584 (A117-S269)

Gene ID
Synonyms
Interleukin-1 beta; IL-1 beta; IL1F2; IL1B; IL-1BETA; IL1F2; IL-1β; IL-1 beta; IL-1B ; Interleukin-1 β; IL-1 β; IL-1β; IL-1 β
AA Sequence

Molecular Weight

Approximately 20.1 kDa

Purity

Greater than 95% as determined by Tris-Bis PAGE. Greater than 95% as determined by HPLC.

Biological Activity
Immobilized Human IL-1 RII hFc at 1 μg/mL (100μL/Well) on the plate. Dose response curve for Biotinylated Human IL-1 Beta His with the EC50 of 9.7 ng/mL determined by ELISA.
Appearance

Lyophilized powder.

Formulation

Lyophilized a 0.22 μm filtered solution of PBS, pH 7.4. Normally 5% trehalose is added as protectant before lyophilization.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Reconstitution

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Storage & Stability

Stored at -20°C. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer. It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Shipping

Room temperature in continental US; may vary elsewhere.

Documentation
  • Reconstitution Calculator

  • Dilution Calculator

  • Specific Active Calculator

The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific active calculator equation

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Active (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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Product Name:
IL-1 beta Protein, Human (Biotinylated, His-Avi)
Cat. No.:
HY-P78143
Quantity:
MCE Japan Authorized Agent: