IL-16 Protein, Human (1203-1332a.a)

IL-16 Protein, Human (1203-1332a.a) is a proinflammatory cytokine containing 130 amino acids.

Recombinant Human IL-16 produced in E. coli is a single, non-glycosylated polypeptide chain containing 130 amino acids. Interleukin-16, a proinflammatory cytokine produced in CD8⁺ lymphocytes, is synthesized as a precursor protein (pro-IL-16). It is postulated that the C-terminal region of pro-IL-16 is cleaved, releasing bioactive IL-16. IL-16 is produced and secreted predominantly by CD8⁺ T lymphocytes, and it exerts chemoattractant, growth factor, and other activities on lymphocytes, monocytes, and eosinophils by interaction with CD4^[1].

Human

E. coli

Tag Free

Q14005 (M1203-S1332)

3603  [NCBI]

MPDLNSSTDSAASASAASDVSVESTAEATVCTVTLEKMSAGLGFSLEGGKGSLHGDKPLTINRIFKGAASEQSETVQPGDEILQLGGTAMQGLTRFEAWNIIKALPDGPVTIVIRRKSLQSKETTAAGDS

18-20 kDa

Greater than 95% as determined by reducing SDS-PAGE.

Lyophilized powder.

Lyophilized after extensive dialysis against 20 mM acetic buffer, pH 5.0, 150 mM NaCl and 0.5 mM DTT.

<1 EU/μg, determined by LAL method.

It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH₂O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).

Stored at -20°C for 2 years. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.

Room temperature in continental US; may vary elsewhere.

• [1]. Zhang Y, et al. Processing and activation of pro-interleukin-16 by caspase-3. J Biol Chem. 1998 Jan 9;273(2):1144-9.  [Content Brief]