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IMPA3 Protein, Mouse (HEK293, His)

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Based on 1 Customer Validation

IMPA3 protein has 3'-nucleotidase activity and can hydrolyze PAP into AMP and phosphate. This function suggests a role in the formation of bone components during endochondral ossification by scavenging PAP produced by Golgi sulfotransferases. IMPA3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IMPA3 protein, expressed by HEK293 , with N-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Mouse
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for IMPA3 Protein, Mouse (HEK293, His)
  • IMPA3 Protein, Mouse (HEK293, His) Product Image
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Tel: 609-228-6898 E-mail: [email protected] [email protected]

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To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
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Biological Activity

Description

IMPA3 protein has 3'-nucleotidase activity and can hydrolyze PAP into AMP and phosphate. This function suggests a role in the formation of bone components during endochondral ossification by scavenging PAP produced by Golgi sulfotransferases. IMPA3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IMPA3 protein, expressed by HEK293 , with N-6*His labeled tag.

Background

IMPA3 protein demonstrates 3'-nucleotidase activity, specifically hydrolyzing adenosine 3',5'-bisphosphate (PAP) into adenosine 5'-monophosphate (AMP) and a phosphate. This enzymatic function suggests a potential role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Notably, IMPA3 exhibits no activity toward 3'-phosphoadenosine 5'-phosphosulfate (PAPS) or inositol phosphate (IP) substrates, including I(1)P, I(1,4)P2, I(1,3,4)P3, I(1,4,5)P3, and I(1,3,4,5)P4. The selective enzymatic profile of IMPA3 underscores its specific involvement in nucleotide metabolism and its potential regulatory role in skeletal development processes.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

MCE Validation Data

  • Purity - SDS-PAGE

    Purity - SDS-PAGE

    ≥ 95%, as determined by reducing SDS-PAGE.

Technical Parameters

  • Species Mouse
  • Source HEK293
  • Tag N-6*His
  • Accession

    Q80V26 (E51-H356)

  • Gene ID
    242291  [NCBI]
  • Molecular Construction
    • N-term
    • 6*His
    • IMPA3 (E51-H356)
      Accession # Q80V26
    • C-term
  • Protein Length

    Partial

  • Synonyms

    BPNT2; Myo-Inositol Monophosphatase A3; Prev. IMPAD1; FLJ20421; IMPA3; Golgi 3-Prime Phosphoadenosine 5-Prime Phosphate 3-Prime Phosphatase; GPAPP; 3'(2'),5'-Bisphosphate Nucleotidase; Golgi-Resident Adenosine 3',5'-Bisphosphate 3'-Phosphatase; Inositol-1

  • AA Sequence

    EVASDGGTVDLREMLAVAVLAAERGGDEVRRVRESNVLHEKSKGKTREGADDKMTSGDVLSNRKMFYLLKTAFPNVQINTEEHVDASDKEVIVWNRKIPEDILKEIAAPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPVLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPKIIVSRSHAGMVKQVALQTFGNQTSIIPAGGAGYKVLALLDVPDMTQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLNGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKSGH

  • Predicted Molecular Mass

    34.3 kDa

  • Molecular Weight

    Approximately 38 kDa, based on SDS-PAGE under reducing conditions.

  • Glycosylation

    Yes

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 10% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

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Dilution Calculator

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The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

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Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • IMPA3 Protein, Mouse (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for IMPA3 Protein, Mouse (HEK293, His)
  • IMPA3 Protein, Mouse (HEK293, His) Product Image