IMPA3 Protein, Mouse (HEK293, His)

IMPA3 protein has 3'-nucleotidase activity and can hydrolyze PAP into AMP and phosphate. This function suggests a role in the formation of bone components during endochondral ossification by scavenging PAP produced by Golgi sulfotransferases. IMPA3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IMPA3 protein, expressed by HEK293 , with N-6*His labeled tag.

IMPA3 protein demonstrates 3'-nucleotidase activity, specifically hydrolyzing adenosine 3',5'-bisphosphate (PAP) into adenosine 5'-monophosphate (AMP) and a phosphate. This enzymatic function suggests a potential role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Notably, IMPA3 exhibits no activity toward 3'-phosphoadenosine 5'-phosphosulfate (PAPS) or inositol phosphate (IP) substrates, including I(1)P, I(1,4)P2, I(1,3,4)P3, I(1,4,5)P3, and I(1,3,4,5)P4. The selective enzymatic profile of IMPA3 underscores its specific involvement in nucleotide metabolism and its potential regulatory role in skeletal development processes.

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Mouse

HEK293

N-6*His

Q80V26 (E51-H356)

242291  [NCBI]

Partial

EVASDGGTVDLREMLAVAVLAAERGGDEVRRVRESNVLHEKSKGKTREGADDKMTSGDVLSNRKMFYLLKTAFPNVQINTEEHVDASDKEVIVWNRKIPEDILKEIAAPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPVLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPKIIVSRSHAGMVKQVALQTFGNQTSIIPAGGAGYKVLALLDVPDMTQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLNGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKSGH

Approximately 38.0 kDa

• 
  ≥ 95%, as determined by reducing SDS-PAGE.

Solution.

Supplied as a 0.22 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 10% glycerol, pH 7.5.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.

<1 EU/μg, determined by LAL method.

N/A.

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping with dry ice.