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  3. IMPA3 Protein, Mouse (HEK293, His)

IMPA3 Protein, Mouse (HEK293, His)

Cat. No.: HY-P70865
COA Handling Instructions

IMPA3 protein has 3'-nucleotidase activity and can hydrolyze PAP into AMP and phosphate. This function suggests a role in the formation of bone components during endochondral ossification by scavenging PAP produced by Golgi sulfotransferases. IMPA3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IMPA3 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of IMPA3 Protein, Mouse (HEK293, His) is 306 a.a., with molecular weight of ~38.0 kDa.

For research use only. We do not sell to patients.

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Description

IMPA3 protein has 3'-nucleotidase activity and can hydrolyze PAP into AMP and phosphate. This function suggests a role in the formation of bone components during endochondral ossification by scavenging PAP produced by Golgi sulfotransferases. IMPA3 Protein, Mouse (HEK293, His) is the recombinant mouse-derived IMPA3 protein, expressed by HEK293 , with N-6*His labeled tag. The total length of IMPA3 Protein, Mouse (HEK293, His) is 306 a.a., with molecular weight of ~38.0 kDa.

Background

IMPA3 protein demonstrates 3'-nucleotidase activity, specifically hydrolyzing adenosine 3',5'-bisphosphate (PAP) into adenosine 5'-monophosphate (AMP) and a phosphate. This enzymatic function suggests a potential role in the formation of skeletal elements derived through endochondral ossification, possibly by clearing adenosine 3',5'-bisphosphate produced by Golgi sulfotransferases during glycosaminoglycan sulfation. Notably, IMPA3 exhibits no activity toward 3'-phosphoadenosine 5'-phosphosulfate (PAPS) or inositol phosphate (IP) substrates, including I(1)P, I(1,4)P2, I(1,3,4)P3, I(1,4,5)P3, and I(1,3,4,5)P4. The selective enzymatic profile of IMPA3 underscores its specific involvement in nucleotide metabolism and its potential regulatory role in skeletal development processes.

Biological Activity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Species

Mouse

Source

HEK293

Tag

N-6*His

Accession

Q80V26 (E51-H356)

Gene ID

242291  [NCBI]

Molecular Construction
N-term
6*His
IMPA3 (E51-H356)
Accession # Q80V26
C-term
Synonyms
Inositol monophosphatase 3; Impad1
AA Sequence

EVASDGGTVDLREMLAVAVLAAERGGDEVRRVRESNVLHEKSKGKTREGADDKMTSGDVLSNRKMFYLLKTAFPNVQINTEEHVDASDKEVIVWNRKIPEDILKEIAAPKEVPAESVTVWIDPLDATQEYTEDLRKYVTTMVCVAVNGKPVLGVIHKPFSEYTAWAMVDGGSNVKARSSYNEKTPKIIVSRSHAGMVKQVALQTFGNQTSIIPAGGAGYKVLALLDVPDMTQEKADLYIHVTYIKKWDICAGNAILKALGGHMTTLNGEEISYTGSDGIEGGLLASIRMNHQALVRKLPDLEKSGH

Molecular Weight

Approximately 38.0 kDa

Purity

Greater than 95% as determined by reducing SDS-PAGE.

Appearance

Solution.

Formulation

Supplied as a 0.2 μm filtered solution of 50 mM Tris-HCl, 150 mM NaCl, 10% Glycerol, pH 7.5.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Documentation

IMPA3 Protein, Mouse (HEK293, His) Related Classifications

Help & FAQs
  • Do most proteins show cross-species activity?

    Species cross-reactivity must be investigated individually for each product. Many human cytokines will produce a nice response in mouse cell lines, and many mouse proteins will show activity on human cells. Other proteins may have a lower specific activity when used in the opposite species.

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The reconstitution calculator equation

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration
= ÷

The dilution calculator equation

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

This equation is commonly abbreviated as: C1V1 = C2V2

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
× = ×
C1   V1   C2   V2

The specific activity calculator equation

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)
Unit/mg = 106 ÷ ng/mL

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IMPA3 Protein, Mouse (HEK293, His)
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HY-P70865
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