Copy Link Print Information

INPP5A Protein, Human (HEK293, His)

Customer Review

Based on 1 Customer Validation

INPP5A Protein, a phosphatase, catalyzes the hydrolysis of inositol phosphates, converting inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. This enzymatic action underscores its role in inositol phosphate metabolism. INPP5A is crucial for the survival of cerebellar Purkinje cells, emphasizing its significance in cellular processes and neuronal function. INPP5A Protein, Human (HEK293, His) is the recombinant human-derived INPP5A protein, expressed by HEK293 , with C-6*His labeled tag.

For research use only. We do not sell to patients.
  • Species: Human
  • Source: HEK293
  • Storage:
    Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.
  • Experimental Validation Results for INPP5A Protein, Human (HEK293, His)
  • INPP5A Protein, Human (HEK293, His) Product Image
Size
Price
Stock
Quantity
Amount: USD 0.00
Please select quantity
or
or

* Please select Quantity before adding items.

To place orders, for customer services and technical support, please contact: MedChemExpress USA

Tel: 609-228-6898 E-mail: [email protected] [email protected]

Service
Data Download

To place orders, for customer services and technical support, please contact:

609-228-6898
[email protected] [email protected]
Find Your Local Distributor Your local distributor: Hycultec GmbHHycultec
  • Biological Activity
  • Technical Parameters
  • Product Properties
  • Documentation
  • Help & FAQs
Top Publications Citing

Use of MCE Products

Biological Activity

Description

INPP5A Protein, a phosphatase, catalyzes the hydrolysis of inositol phosphates, converting inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. This enzymatic action underscores its role in inositol phosphate metabolism. INPP5A is crucial for the survival of cerebellar Purkinje cells, emphasizing its significance in cellular processes and neuronal function. INPP5A Protein, Human (HEK293, His) is the recombinant human-derived INPP5A protein, expressed by HEK293 , with C-6*His labeled tag.

Background

INPP5A Protein is a phosphatase with a specific enzymatic activity, catalyzing the hydrolysis of the 5-phosphate of inositol 1,4,5-trisphosphate to inositol 1,4-bisphosphate, and inositol 1,3,4,5-tetrasphosphate to inositol 1,3,4-trisphosphate. This enzymatic action highlights its role in inositol phosphate metabolism. Notably, INPP5A plays a crucial and essential role in the survival of cerebellar Purkinje cells, emphasizing its significance in cellular processes and neuronal function.

Verified Bioactivity

The enzyme activity of this recombinant protein is testing in progress, we cannot offer a guarantee yet.

Technical Parameters

  • Species Human
  • Source HEK293
  • Tag C-6*His
  • Accession

    Q14642 (M1-V410)

  • Gene ID
    3632  [NCBI]
  • Molecular Construction
    • N-term
    • INPP5A (M1-V410)
      Accession # Q14642
    • 6*His
    • C-term
  • Protein Length

    Full Length of Inositol polyphosphate-5-phosphatase A Chain

  • Synonyms

    INPP5A; InsP3 5-Phosphatase; Inositol Polyphosphate-5-Phosphatase A; Type I Inositol-1,4,5-Trisphosphate 5-Phosphatase; 5PTASE; Type I Inositol-1,4,5-Trisphosphate 5-Phophatase; Type I Inositol 1,4,5-Trisphosphate 5-Phosphatase; Inositol Polyphosphate 5-P

  • AA Sequence

    MAGKAAAPGTAVLLVTANVGSLFDDPENLQKNWLREFYQVVHTHKPHFMALHCQEFGGKNYEASMSHVDKFVKELLSSDAMKEYNRARVYLDENYKSQEHFTALGSFYFLHESLKNIYQFDFKAKKYRKVAGKEIYSDTLESTPMLEKEKFPQDYFPECKWSRKGFIRTRWCIADCAFDLVNIHLFHDASNLVAWETSPSVYSGIRHKALGYVLDRIIDQRFEKVSYFVFGDFNFRLDSKSVVETLCTKATMQTVRAADTNEVVKLIFRESDNDRKVMLQLEKKLFDYFNQEVFRDNNGTALLEFDKELSVFKDRLYELDISFPPSYPYSEDARQGEQYMNTRCPAWCDRILMSPSAKELVLRSESEEKVVTYDHIGPNVCMGDHKPVFLAFRIMPGAGKPHAHVHKCCV

  • Molecular Weight

    Approximately 45 kDa, based on SDS-PAGE under reducing conditions.

  • Purity

    ≥ 95%, as determined by reducing SDS-PAGE.

Product Properties

Appearance

Solution

Formulation

1.Supplied as a 0.22 μm filtered solution of 20 mM PB, 150 mM NaCl, pH 7.4.
2.Supplied as a 0.22 μm filtered solution in 500 mM Tris-HCl, 10 mM MgCl2, 100 mM KCl, pH 7.5.
3.Supplied as a 0.22 μm filtered solution in 50 mM Tris-HCl, 10 mM MgCl2, 100 mM KCl, pH 7.5.
Please refer to the lot-specific COA for specific buffer information.
Note: If this product is used in SPR assay, please note that the protein buffer should not contain primary amino components (such as Tris and Imidazole), and the buffer needs to be replaced.

Endotoxin Level

<1 EU/μg, determined by LAL method.

Storage & Stability

Stored at -80°C for 1 year from date of receipt. It is stable at -20°C for 3 months after opening. It is recommended to freeze aliquots at -80°C for extended storage. Avoid repeated freeze-thaw cycles.

Shipping

Shipping with dry ice.

Calculators

Reconstitution Calculator

Volume (to add to vial) = Mass (in vial) ÷ Desired Reconstitution Concentration

Volume (to add to vial) Volume (to add to vial)
=
Mass (in vial) Mass (in vial)
÷
Desired Reconstitution Concentration Desired Reconstitution Concentration
Dilution Calculator

Concentration (start) × Volume (start) = Concentration (final) × Volume (final)

Concentration (start) Concentration (start)
×
Volume (start) Volume (start)
=
Concentration (final) Concentration (final)
×
Volume (final) Volume (final)
The Specific Activity Calculator Equation
  • Specific Activity (Unit/mg)
  • Biological Activity (ED50)

Specific Activity (Unit/mg) = 106 ÷ Biological Activity (ED50)

Specific Activity (Unit/mg) Specific Activity (Unit/mg)
Unit/mg
= 106 ÷
Biological Activity (ED50) Biological Activity (ED50)
106 ÷
ng/mL
MOQ
Minimum order quantity
100 mg

Get Quote In-stock

Other size
Get Quote
Please select quantity
Amount: USD 0.00

Measured by its ability to inhibit anti-CD3-induced proliferation of stimulated CTLL-2 mouse cytotoxic T cells. The ED50 this effect is 0.8023 μg/ml, corresponding to a specific activity is 1246.42 units/mg.

Assay Types
  • 1/16

    MedChemExpress Validation
     ≥ 95%, as determined by reducing SDS-PAGE.

  • INPP5A Protein, Human (HEK293, His) Product Image
    1/16
  • Experimental Validation Results for INPP5A Protein, Human (HEK293, His)
  • INPP5A Protein, Human (HEK293, His) Product Image