Integrin alpha 2 beta 1 Protein, Human (E534K, HEK293, His)
Based on 1 Customer Validation
- Species: Human
- Source: HEK293
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Storage:Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
To place orders, for customer services and technical support, please contact: MedChemExpress USA
Tel: 609-228-6898 E-mail: [email protected] [email protected]
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Biological Activity
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Technical Parameters
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Product Properties
- Documentation
- Help & FAQs
Biological Activity
Verified Bioactivity
Measured by its binding ability in a functional ELISA. Immobilized Human Collagen I protein at 2 μg/mL (100 μL/well) can bind Human ITGA2 & ITGB1 Heterodimer Protein with a linear range of 0.078-2.5 μg/mL.
Technical Parameters
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Species Human
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Source HEK293
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Tag C-His
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Accession
P17301-1 (Y30-T1132,E534K)&P05556 (Q21-D728)
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Gene ID3673&3688
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Molecular Construction
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N-term
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ITGA2 (Y30-T1132,E534K)
Accession # P17301-1 -
His
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C-term
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N-term
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ITGB1 (Q21-D728)
Accession # P05556 -
C-term
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Synonyms
ITGA2; Alpha 2 Integrin; Prev. CD49B; Very Late Activation Protein 2 Receptor, Alpha-2 Subunit; GPIa; Very Late Activation Receptor Alpha-2 Subunit; CD49 Antigen-Like Family Member B; Human Platelet Alloantigen System 5; Platelet Membrane Glycoprotein Ia;
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Predicted Molecular Mass
127.6 kDa (ITGA2) & 83.7 kDa (ITGB1)
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Molecular Weight
Approximately 150-180 kDa (ITGA2) & 100-120 kDa (ITGB1), based on SDS-PAGE under non reducing (N) conditions, due to the glycosylation.
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Glycosylation
Yes
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Structure/Form
Heterodimer
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Purity
≥ 95%, as determined by reducing SDS-PAGE.
Product Properties
Lyophilized powder.
Lyophilized from 0.22 μm filtered solution of 50 mM Tris, 150 mM NaCl, pH 7.5 with 10% trehalose as protectant.
Note: For SPR assay, please replace the buffer. Primary amine components (e.g., Tris, imidazole) can affect protein-coupled chips.
<1 EU/μg, determined by LAL method.
It is not recommended to reconstitute to a concentration less than 100 μg/mL in ddH2O. For long term storage it is recommended to add a carrier protein (0.1% BSA, 5% HSA, 10% FBS or 5% Trehalose).
Stored at -20°C for 2 years from date of receipt. After reconstitution, it is stable at 4°C for 1 week or -20°C for longer (with carrier protein). It is recommended to freeze aliquots at -20°C or -80°C for extended storage.
Room temperature in continental US; may vary elsewhere.
Documentation
Calculators
Concentration (start) × Volume (start) = Concentration (final) × Volume (final)
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